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Tricarboxylic Acid (TCA) Cycle Enzymes and Intermediates Modulate Intracellular Cyclic di-GMP Levels and the Production of Plant Cell Wall-Degrading Enzymes in Soft Rot Pathogen Dickeya dadantii

文献类型： 外文期刊

作者： Yuan, Xiaochen 1 ; Zeng, Quan 3 ; Xu, Jingsheng 4 ; Severin, Geoffrey B. 5 ; Zhou, Xiang 6 ; Waters, Christopher M. 7 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Sci & Technol, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety State Key Lab, Nanjing 210014, Peoples R China

2.Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA

3.Connecticut Agr Expt Stn, Dept Plant Pathol & Ecol, New Haven, CT 06511 USA

4.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

5.Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

6.Zhejiang Agr & Forestry Univ, Sch Forestry & Biotechnol, Hangzhou 311300, Peoples R China

7.Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

8.Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA

9.ARS, US Salin Lab, USDA, Riverside, CA 92507 USA

关键词： bacterial pathogenesis; c-di-GMP; cell wall; Dickeya dadantii; metabolism; pectate lyase; soft rot; TCA cycle

期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：4.171； 五年影响因子：4.836 ）

ISSN： 0894-0282

年卷期： 2020 年 33 卷 2 期

页码：

收录情况： SCI

摘要： Dickeya dadantii is a plant-pathogenic bacterium that causes soft-rot in a wide range of plants. Although we have previously demonstrated that cyclic bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP), a bacterial secondary messenger, plays a central role in virulence regulation in D. dadantii, the upstream signals that modulate c-di-GMP remain enigmatic. Using a genome-wide transposon mutagenesis approach of a Delta hfq mutant strain that has high c-di-GMP and reduced motility, we uncovered transposon mutants that recovered the c-di-GMP-mediated repression on swimming motility. A number of these mutants harbored transposon insertions in genes encoding tricarboxylic acid (TCA) cycle enzymes. Two of these TCA transposon mutants were studied further by generating chromosomal deletions of the fumA gene (encoding fumarase) and the sdhCDAB operon (encoding succinate dehydrogenase). Disruption of the TCA cycle in these deletion mutants resulted in reduced intracellular c-di-GMP and enhanced production of pectate lyases (Pels), a major plant cell wall-degrading enzyme (PCWDE) known to be transcriptionally repressed by c-diGMP. Consistent with this result, addition of TCA cycle intermediates such as citrate also resulted in increased c-di-GMP levels and decreased production of Pels. Additionally, we found that a diguanylate cyclase GcpA was solely responsible for the observed citrate-mediated modulation of c-di-GMP. Finally, we demonstrated that addition of citrate induced not only an overproduction of GcpA protein but also a concomitant repression of the c-di-GMP-degrading phosphodiesterase EGcpB which, together, resulted in an increase in the intracellular concentration of c-di-GMP. In summary, our report demonstrates that bacterial respiration and respiration metabolites serve as signals for the regulation of c-di-GMP signaling.

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