文献类型： 外文期刊
作者： Wang, Guixing 1 ; Zhang, Xiaoyan 2 ; Sun, Zhaohui 2 ; Zhao, Yaxian 2 ; Du, Wei 2 ; Cui, Jianbin 3 ; Hou, Jilun 2 ; Wang, 1 ;
作者机构： 1.Minist Agr, Key Lab Aqut Genom, Beijing 100141, Peoples R China
2.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Hebei, Peoples R China
3.Agr Univ Hebei, Ocean Coll, Qinhuangdao 066009, Hebei, Peoples R China
关键词： gynogenesis; polyploidy; chromosome manipulation; mosaic
期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）
ISSN： 2096-5508
年卷期： 2020 年 38 卷 1 期
页码：
收录情况： SCI
摘要： Tetraploid fish are important for mass production of triploids in polyploid breeding. In this study, we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder Paralichthys olivaceus. The induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream Pagrus major, administering a cold shock (0 degrees C, 5 to 45 min) 3 min after fertilization to inhibit second polar body exclusion, incubating the eggs for 60 min at 17 degrees C, and treating them with a 650 kg/cm(2) hydrostatics pressure shock for 6 min. We named the embryos gyno-tetraploids that developed from eggs after such treatments. The hatching rate of the gyno-tetraploids ranged from 20.99%+/- 3.66% to 36.01%+/- 2.79%, and the tetraploid rate ranged from 80.00% to 100.00%. All-maternal inheritance was verified using 6 high-recombination-rate microsatellite markers. This method successfully induced gyno-tetraploidy. The successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.
- 相关文献
作者其他论文 更多>>
-
Identification candidate genes and loci associated with hypermelanosis of the blind side in Japanese flounder ( Paralichthys olivaceus) ) using the combination of RNA-Seq and BSA-Seq
作者：Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Zhang, Jian;Fang, Xiao
关键词：Recombinant doubled haploid; Paralichthys olivaceus; BSA-seq; Hypermelanosis; Transcriptomic analysis
-
Genetic diversity study of self-fertilized and gynogenetic Japanese flounder ( Paralichthys olivaceus) in whole genomic level
作者：San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Wang, Yufen;Hou, Jilun;San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Ren, Yuqin;Wang, Yufen;Hou, Jilun;San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Ren, Yuqin;Wang, Yufen;Hou, Jilun
关键词：Self-fertilization; Meiotic gynogenesis; Mitotic gynogenesis; Single nucleotide polymorphism; Genetic diversity
-
Effect of Temperature on the Early Development of Paralichthys olivaceus Otoliths
作者：Meng, Gao;Tang, Jiabao;Sun, Zhaohui;Si, Fei;Meng, Gao;Tang, Jiabao;Sun, Zhaohui;Si, Fei;Meng, Gao;Sun, Zhaohui;Si, Fei;Tang, Jiabao;Wang, Qinglin;Yu, Shanshan;Wang, Qinglin;Yu, Shanshan
关键词：
Paralichthys olivaceus ; otoliths; development; temperature -
Toxic effects of anionic polyacrylamide on the developmental stages of Oryzias melastigma embryos and larvae
作者：Zhao, Xinya;Ren, Hai;Fu, Huiru;Jin, Xiaomin;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei
关键词：Anionic polyacrylamide; Oryzias melastigma; Oxidative stress; Cardiotoxicity; Developmental toxicity
-
Community Structure and Influencing Factors of Macro-Benthos in Bottom-Seeded Marine Pastures: A Case Study of Caofeidian, China
作者：Xue, Xiangping;Sun, Zhaohui;Zan, Jiangwei;Xu, Xinjing;Liu, Xia;Gao, Song;Si, Fei;Xue, Xiangping;Sun, Zhaohui;Zan, Jiangwei;Xu, Xinjing;Liu, Xia;Gao, Song;Si, Fei;Yun, Long;Wang, Guangyu;Liu, Mingshuai
关键词：marine pastures; benthic organisms; community structure; environmental factors
-
Effect of chronic heat stress in roughskin sculpin (Trachidermus fasciatus)
作者：Liu, Zitong;Liu, Zitong;San, Lize;He, Zhongwei;Liu, Yufeng;Han, Tian;Ren, Jiangong;Ren, Yuqin;Hou, Jilun;Liu, Zitong;San, Lize;He, Zhongwei;Liu, Yufeng;Han, Tian;Ren, Jiangong;Ren, Yuqin;Hou, Jilun;Han, Tian;Gong, Chunguang
关键词：Roughskin sculpin; Heat stress; Gill tissue structure; Heat stress protein
-
Isolation, identification and the pathogenicity characterization of Pseudomonas putida 1C3 and its activation on immune responses in Japanese flounder (Paralichthys olivaceus)
作者：Zhao, Yaxian;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Ren, Jiangong;Zhang, Yitong;Wang, Yufen;Wang, Guixing;San, Lize;Hou, Jilun;Zhao, Yaxian;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Ren, Jiangong;Zhang, Yitong;Wang, Yufen;Wang, Guixing;San, Lize;Hou, Jilun;Zhao, Yaxian;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Ren, Jiangong;Zhang, Yitong;Wang, Yufen;Wang, Guixing;San, Lize;Hou, Jilun
关键词：Paralichthys olivaceus; Pseudomonas putida; Multidrug-resistant; Pathogenicity; Transcriptome
