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Insights into the venom protein components of the egg parasitoid Anastatus japonicus (Hymenoptera: Eupelmidae)

文献类型： 外文期刊

作者： Wang, Chengxing 1 ; Jin, Fengliang 1 ; De Mandal, Surajit 1 ; Zeng, Lu 1 ; Zhang, Yuxin 1 ; Hua, Yanyan 1 ; Hong, Ying 1 ;

作者机构： 1.South China Agr Univ, Coll Agr, Dept Entomol, Lab Biopesticide Creat & Applicat Guangdong Prov, Guangzhou, Peoples R China

2.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

关键词： venom proteins; Illumina sequencing; proteomics; RT-qPCR; A; japonicus

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期：

页码：

收录情况： SCI

摘要： BACKGROUND Parasitoid venom is composed of a complex mixture of various active substances with different biological functions and is injected in the host during the parasitoid oviposition. Anastatus japonicus (Hymenoptera: Eupelmidae) is an egg parasite of Tessaratoma papillosa (Hemiptera: Tessaratomidae). Although the venom of this egg parasitoid plays an important role in the parasitic process, relatively little work has been done to address the mechanism. RESULTS In the present study, proteomic analysis was performed to identify the proteins that play an important role in the parasitic process of A. japonicus. A total of 2084 proteins were identified, including 81 putative venom proteins, most of which were identified as Hexamerin, Chitinase 2, Calreticulin, Heat shock protein 83-like, Serine protease, Arginine kinase, Phosphoserine aminotransferase and Actin protein. Together the before (Be) and after (Af) parasitization venom contains 1628 proteins, including 212 DEPs with 181 and 31 significantly up-regulated and down-regulated respectively. In addition, 10 differentially expressed proteins (DEPs) with fold change >= 8.71 were subjected to RT-qPCR to validate the proteomic data. The differential expression analysis revealed that nine proteins were specifically present in the pre-parasitic venom, whereas 26 proteins were specific to the post-parasitic treatments. Results of RT-qPCR analysis showed high expression of the selected DEPs which further validated our proteomics data. CONCLUSION These new proteomic data greatly enrich our current knowledge about key venom proteins associated with parasitic process in A. japonicus and contribute to better understanding of the parasitic mechanisms leading to the development of new biological control strategies. (c) 2020 Society of Chemical Industry

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