文献类型： 外文期刊
作者： Du, Minmin 1 ; Zhou, Ke 1 ; Liu, Yuanyuan 3 ; Deng, Lei 1 ; Zhang, Xiaoyue 1 ; Lin, Lihao 1 ; Zhou, Ming 2 ; Zhao, Wei 1 ; W 1 ;
作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, State Key Lab Plant Genom, Beijing 100101, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China
3.Zhejiang Agr & Forestry Univ, Coll Agr & Food Sci, Key Lab Qual Improvement Agr Prod Zhejiang Prov, Hangzhou 311300, Peoples R China
关键词： Solanum lycopersicum; CRISPR; Cas9; male sterility; colour-maintainer; hybrid seed production; technical advance
期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）
ISSN： 0960-7412
年卷期：
页码：
收录情况： SCI
摘要： Incorporating male sterility into hybrid seed production reduces its cost and ensures high varietal purity. Despite these advantages, male-sterile lines have not been widely used to produce tomato (Solanum lycopersicum) hybrid seeds. We describe the development of a biotechnology-based breeding platform that utilized genic male sterility to produce hybrid seeds. In this platform, we generated a novel male-sterile tomato line by clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated mutagenesis of a stamen-specific gene SlSTR1 and devised a transgenic maintainer by transforming male-sterile plants with a fertility-restoration gene linked to a seedling-colour gene. Offspring of crosses between a hemizygous maintainer and the homozygous male-sterile plant segregated into 50% non-transgenic male-sterile plants and 50% male-fertile maintainer plants, which could be easily distinguished by seedling colour. This system has great practical potential for hybrid seed breeding and production as it overcomes the problems intrinsic to other male-sterility systems and can be easily adapted for a range of tomato cultivars and diverse vegetable crops.
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