文献类型： 外文期刊
作者： Li Zhaolong 1 ; Fu, Guanghua 1 ; Feng, Zhihua 1 ; Chen, Jianhua 1 ; Shi, Shaohua 1 ; Liu, Rongchang 1 ; Cheng, Longfei 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peoples R China
3.Fujian Anim Dis Control Technol Ctr, Fuzhou 350013, Fujian, Peoples R China
关键词： DuCV; Peripheral blood mononuclear cells; Inactivated vaccine; Muscovy ducks
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）
ISSN： 0378-1135
年卷期： 2020 年 241 卷
页码：
收录情况： SCI
摘要： Duck circovirus (DuCV), an immunosuppressive pathogen, causes serious damage to waterfowls worldwide. A highly efficient vaccine would play a crucial role in preventing DuCV infections in the waterfowl breeding industry. However, to date, there is a dearth of commercial vaccines owing to the lack of a cell culture system for propagating the requisite virus amounts in vitro. In this study, we isolated DuCVs from Muscovy ducks, helped them proliferate using peripheral blood mononuclear cells (PBMCs), and developed an inactivated vaccine. Muscovy ducks vaccinated with the inactivated vaccine had higher neutralizing antibody titers than the control ducks and higher protection in the challenge experiment (as assessed by weight measurement). Moreover, the inactivated vaccine did not cause feather abnormalities, growth repression, and dwarf syndrome; likewise, lesions and lymphocyte apoptosis in the bursa of Fabricius, spleen, and thymus were not observed. Significantly lower virus shedding from the inactivated vaccine was detected up to 42 days post-inoculation. Together, these results suggest that the inactivated DuCV vaccine can induce a high immune response, is relatively safer for Muscovy ducks, and thus it is a protective vaccine candidates against DuCV infection.
- 相关文献
作者其他论文 更多>>
-
Specific detection of pigeon parvovirus with TaqMan real-time PCR technology
作者：Chen, Cuiteng;Zhu, Chunhua;Chen, Shuyu;Chen, Zhen;Fu, Huanru;Chen, Yuyi;Zhang, Mengyan;Zhang, Wenyu;Huang, Yu;Cheng, Longfei;Wan, Chunhe;Chen, Shuyu;Fu, Huanru;Zhang, Wenyu;Chen, Yuyi;Zhang, Mengyan
关键词：Pigeon parvovirus (PiPV); NS; TaqMan-PCR; Epidemiological investigation
-
Epidemiological investigation of goose circovirus based on a newly developed indirect ELISA method
作者：Chen, Jialong;Xue, Wenchang;Yao, Zhanxin;Wang, Chao;Wang, He;Zhang, Jipei;Chen, Jidang;Zhu, Wanjun;Wang, He;Tang, Yi;Liu, Rongchang;Chen, Jidang
关键词：goose circovirus; capsid protein; serological detection methods; prokaryotic expression; indirect ELISA
-
Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis
作者：Dong, Qishan;Lu, Lizhi;Gu, Tiantian;Tian, Yong;Zong, Yibo;Zeng, Tao;Dong, Qishan;He, Jun;Lu, Lizhi;Gu, Tiantian;Tian, Yong;Zong, Yibo;Zeng, Tao;Sun, Jing;Huang, Yu;Fu, Qiuling
关键词：Volatile flavor; Duck eggs; Lipidomics; Fermented feed; Off-odor
-
Characterization of a Capsule-Deficient Pasteurella multocida Isolated from Cygnus melancoryphus: Genomic, Phenotypic, and Virulence Insights
作者：Jiang, Nansong;Chen, Hongmei;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Cheng, Longfei;Jiang, Nansong;Chen, Hongmei;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Cheng, Longfei;Jiang, Nansong;Chen, Hongmei;Wang, Weiwei;Liang, Qizhang;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Cheng, Longfei
关键词：
Pasteurella multocida ;Cygnus melancoryphus ; capsule deficiency; FCF147 strain; adaptation; virulence -
Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection
作者：Feng, Zhihua;Chen, Jianxin;Chen, Qi;Feng, Zhihua;Chen, Jianxin;Chen, Qi;Feng, Zhihua;Fu, Yajuan;Yang, Sheng;Zhao, Heng;Lin, Minhua;Liu, Chuancheng;Huang, Weili;He, Xinyan;Chen, Yao;Shen, Yangkun;Chen, Qi;Feng, Zhihua;Fu, Yajuan;Yang, Sheng;Zhao, Heng;Lin, Minhua;Liu, Chuancheng;Huang, Weili;He, Xinyan;Chen, Yao;Shen, Yangkun;Chen, Qi;Li, Zhaolong
关键词：Swine alpha-coronaviruses; Host factor; Mouse model; Antibody drug; Cross-species transmission
-
Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7-and pH-dependent manner
作者：Liang, Qi-Zhang;Chen, Wei;Huang, Yu;Ji, Chun-Miao;Wang, Bin;Huang, Yao-Wei;Ji, Chun-Miao;Cong, Feng;Wang, Bin;Huang, Yao-Wei;Huang, Yao-Wei
关键词：Avian deltacoronavirus; porcine deltacoronavirus; entry pathway; pseudovirus; endocytosis
-
The Infectivity and Pathogenicity Characteristics of a Recombinant Porcine Epidemic Diarrhea Virus, CHFJFQ
作者：Feng, Zhihua;Chen, Qi;Feng, Zhihua;Zhao, Heng;Lin, Minhua;Huang, Weili;Liu, Chuancheng;Shen, Yangkun;Chen, Qi;Feng, Zhihua;Zhao, Heng;Lin, Minhua;Huang, Weili;Liu, Chuancheng;Shen, Yangkun;Chen, Qi;Feng, Zhihua;Chen, Qi;Li, Zhaolong
关键词：PEDV; origin; infection; cross-species transmission
