文献类型： 外文期刊
作者： Li Zhaolong 1 ; Fu, Guanghua 1 ; Feng, Zhihua 1 ; Chen, Jianhua 1 ; Shi, Shaohua 1 ; Liu, Rongchang 1 ; Cheng, Longfei 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Prov Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Fujian, Peoples R China
3.Fujian Anim Dis Control Technol Ctr, Fuzhou 350013, Fujian, Peoples R China
关键词： DuCV; Peripheral blood mononuclear cells; Inactivated vaccine; Muscovy ducks
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）
ISSN： 0378-1135
年卷期： 2020 年 241 卷
页码：
收录情况： SCI
摘要： Duck circovirus (DuCV), an immunosuppressive pathogen, causes serious damage to waterfowls worldwide. A highly efficient vaccine would play a crucial role in preventing DuCV infections in the waterfowl breeding industry. However, to date, there is a dearth of commercial vaccines owing to the lack of a cell culture system for propagating the requisite virus amounts in vitro. In this study, we isolated DuCVs from Muscovy ducks, helped them proliferate using peripheral blood mononuclear cells (PBMCs), and developed an inactivated vaccine. Muscovy ducks vaccinated with the inactivated vaccine had higher neutralizing antibody titers than the control ducks and higher protection in the challenge experiment (as assessed by weight measurement). Moreover, the inactivated vaccine did not cause feather abnormalities, growth repression, and dwarf syndrome; likewise, lesions and lymphocyte apoptosis in the bursa of Fabricius, spleen, and thymus were not observed. Significantly lower virus shedding from the inactivated vaccine was detected up to 42 days post-inoculation. Together, these results suggest that the inactivated DuCV vaccine can induce a high immune response, is relatively safer for Muscovy ducks, and thus it is a protective vaccine candidates against DuCV infection.
- 相关文献
作者其他论文 更多>>
-
Codon usage bias of goose circovirus and its adaptation to host
作者：Xu, Quanming;Cao, Jie;Wan, Chunhe;Rai, Kul Raj;Zhu, Binling;Liu, Dan
关键词：goose circovirus; codon usage bias; natural selection; mutation; host adaptability
-
Rapid detection of pigeon adenovirus 2 using a TaqMan real-time PCR assay
作者：Chen, Cuiteng;Zhu, Chunhua;Chen, Zhen;Cai, Guozhang;Lin, Lin;Zhang, Shizhong;Jiang, Bin;Miao, Zhongwei;Fu, Guanghua;Huang, Yu;Wan, Chunhe
关键词：pigeon adenovirus 2; fiber 2; TaqMan-qPCR assay; YPDS; epidemiological surveillance
-
Genomic analysis reveals the population structure and antimicrobial resistance of avian Pasteurella multocida in China
作者：Jiang, Nansong;Chen, Hongmei;Cheng, Longfei;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Jiang, Nansong;Chen, Hongmei;Cheng, Longfei;Fu, Qiuling;Liu, Rongchang;Liang, Qizhang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Jiang, Nansong;Chen, Hongmei;Cheng, Longfei;Fu, Qiuling;Liu, Rongchang;Liang, Qizhang;Fu, Guanghua;Wan, Chunhe;Huang, Yu
关键词：
-
Inhibitory mechanisms of decoy receptor 3 in cecal ligation and puncture-induced sepsis
作者：Su, Jingqian;Chen, Wenzhi;Zhou, Fen;Tong, Zhiyong;Wu, Shun;Guan, Biyun;Feng, Zhihua;Chen, Kunsen;Chen, Qi;Chen, Wenzhi;Li, Rui;Ye, Zhen;Zhang, Yichao;Lin, Ben;Chen, Long;Yu, Xing
关键词：gut microbiota; decoy receptor 3; sepsis; anti-inflammatory
-
Simultaneous detection and differentiation of DuCV-1 and DuCV-2 by high-resolution melting analysis
作者：Fu, Huanru;Zhao, Min;Chen, Shuyu;Huang, Yu;Wan, Chunhe;Fu, Huanru;Zhao, Min;Chen, Shuyu
关键词：duck circovirus; DuCV-1; DuCV-2; differentiation; high-resolution melting
-
Gp85 protein encapsulated by alginate-chitosan composite microspheres induced strong immunogenicity against avian leukosis virus in chicken
作者：Lei, Tianyu;Zhuang, Liyun;Dai, Tingting;Meng, Qingfu;Zhang, Xiaodong;Bao, Yinli;Huang, Cuiqin;Lin, Weiming;Zheng, Xintian;Lei, Tianyu;Zhuang, Liyun;Dai, Tingting;Zhang, Xiaodong;Zheng, Xintian;Liu, Rongchang;Huang, Yu;Meng, Qingfu;Bao, Yinli;Huang, Cuiqin;Lin, Weiming;Zheng, Xintian
关键词：avian leukosis virus subgroup J; Gp85 protein; alginate-chitosan composite microspheres; vaccine; immunogenicity
-
Hypervirulent fowl adenovirus serotype 4 elicits early innate immune response and promotes virus-induced cellular autophagy in the spleen
作者：Zhu, Chunhua;Zhou, Jiayu;Chen, Zhen;Chen, Cuiteng;Wan, Chunhe;Huang, Yu
关键词：hypervirulent fowl adenovirus serotype 4; TBK1/IRF7 signaling pathway; autophagy; spleen
