Identification and characterization of interferon regulatory factor 1 from Lateolabrax japonicus involved in antiviral immune response against grouper nervous necrosis virus infection
文献类型： 外文期刊
作者： Ma, Zhenhua 1 ; Chen, Xu 1 ; Yang, Rui 1 ; Hu, Jing 1 ; Zhou, Shengjie 1 ; Yang, Qibing 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Trop Aquaculture Res & Dev Ctr, South China Sea Fisheries Res Inst, Sanya 572018, Peoples R China
2.Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Peoples R China
关键词： Lateolabrav japonicas; LjIRF-1; LjIFN-1; GNNV; Poly (I:C)
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2020 年 97 卷
页码：
收录情况： SCI
摘要： Interferon regulatory factors (IRFs) play a key role in mediating the host response against pathogen infection and other important biological processes. In the present study, an interferon regulation factor 1 gene was identified from Lateolabrax japonicus (designated LjIRF-1), the cDNA sequence of LjIRF-1 was 1394 bp long, and with an open reading frame (ORF) of 945 bp that encodes a peptide of 314 amino acids. Bioinformatics data showed that LjIRF-1 possesses a DNA-binding domain (DBD) and two low complexity regions, which shared 56-81% identity to other fish IRF-1s. The LjIRF-1 transcripts were detectable in all examined tissues of healthy L. japonicus, with higher levels in the blood, head-kidney, intestine, gill and spleen. When challenged with grouper nervous necrosis virus (GNNV) and poly (I:C) infection, both the mRNA expression levels of LjIRF-1 and L. japonicus interferon-1 gene (designated LjIFN-1) were significantly up-regulated. Furthermore, like with poly (I:C), the active purified recombinant protein (rLjIRF-1) was also capable of increasing the expression level of LjIFN-1; controlling the copy number of GNNV under lethiferous titer (10(11)-10(12) copies/mu L) and promoting the survival rate of GNNV infected L. japonicas. Combine all the results, we deduced that LjIRF-1 is involved in defending GNNV infection by simulating LjIFN-1 signal pathway in L. japonicas.
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