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Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of an immersion DNA vaccine against infectious spleen and kidney necrosis virus in mandarin fish

文献类型： 外文期刊

作者： Zhao, Zhao 2 ; Zhang, Chen 2 ; Lin, Qiang 1 ; Li, Ning-Qiu 1 ; Huang, Zhi-Bin 1 ; Zhao, Mi 3 ; Fu, Xiao-Zhe 1 ; Wang, Gao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs,Key Lab Aquat Anim Imm, Guangzhou 510380, Peoples R China

2.Northwest A&F Univ, Coll Anim Sci & Technol, Xinong Rd 22nd, Yangling 712100, Shaanxi, Peoples R China

3.Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China

4.Sun Yat Sen Univ, Guangdong Prov Engn Technol Res Ctr Hlth Breeding, Sch Life Sci, Guangzhou 510275, Peoples R China

关键词： Infectious spleen and kidney necrosis virus; DNA vaccine; Single-walled carbon nanotubes; Mandarin fish

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2020 年 97 卷

页码：

收录情况： SCI

摘要： As a high mortality disease, Infectious spleen and kidney necrosis virus (ISKNV) can cause massive economic damage on mandarin fish farming industry in China, which seriously hindered the development of mandarin fish farming industry. In this research, SWCNTs (single-walled carbon nanotubes) as a candidate for DNA vaccine carrier was vaccinated by immersion (1, 2, 5, 10, 20 mg/L) in juvenile mandarin fish. In muscle, spleen and kidney tissues, the results showed that transcription and expression of MCP gene can be detected in pcDNA-MCP and SWCNTs-pcDNA-MCP groups after bath immunization. The immune response (immune-related genes expression, serum antibody production, enzyme activities and C3 content) was significantly enhanced in fish which vaccinated with SWCNTs-pcDNA-MCP in comparison with those vaccinated with pcDNA-MCP alone. After 14 d challenge, the RPS (relative percentage survival) can be enhanced which using SWCNTs as a carrier in SWCNTs-pcDNA-MCP (82.4%) group at 20 mg/L (the highest vaccine dose) than the naked pcDNA-MCP (54.2%) group. This study reveals that functionalized SWCNTs could be a promising immersion DNA vaccine carrier in aquaculture.

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