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Active immunization against AMH reveals its inhibitory role in the development of pre-ovulatory follicles in Zhedong White geese

文献类型： 外文期刊

作者： Chen, R. 1 ; Dai, Z. C. 1 ; Zhu, H. X. 1 ; Lei, M. M. 1 ; Li, Y. 3 ; Shi, Z. D. 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China

2.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety State Key Lab, Nanjing 210014, Peoples R China

3.Jurong Anim Dis Prevent & Control Ctr, Jurong 212400, Peoples R China

关键词： Anti-mullerian hormone; Immunization; Ovarian follicular development; Egg laying; Geese

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2020 年 144 卷

页码：

收录情况： SCI

摘要： The aim of this study was to investigate the effects of active immunization against recombinant Anti-Mullerian hormone (AMH) protein on the ovarian follicular development, egg production, and molecular regulatory mechanisms in broody-prone Zhedong White geese. For this, a recombinant goose AMH protein was expressed using a prokaryotic expression system. Fifty incubating geese from the same genetic background were selected and equally divided into two groups. The immunization group was actively immunized against the recombinant goose AMH protein, whereas the control group was immunized against bovine serum albumin (BSA). Immunization against AMH accelerated ovarian follicular development and increased clutch sizes by one to two eggs in two consecutive laying-incubation cycles. Furthermore, immunization against AMH upregulated the mRNA transcription levels of the FSH-beta gene in the pituitary gland, and FSHR, 3beta-HSD, and Smad4 genes in the granulosa layer of pre-ovulatory follicles; however, immunization downregulated the expression of the OCLN gene in the granulosa layer of pre-ovulatory follicles, and Smad5 and Smad9 genes in the granulosa layer of SYFs. These results suggest that AMH might hinder ovarian follicular development by decreasing both pituitary FSH secretion as well as ovarian follicular sensitivity to FSH. The latter molecular mechanism could be fulfilled by regulating Smad5 or Smad9 signals in SYFs, as well as the FSHR and Smad4 signals that affect progesterone synthesis and yolk deposition in the pre-ovulatory follicles. (C) 2020 Elsevier Inc. All rights reserved.

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