Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
文献类型： 外文期刊
作者： Dai, G. 1 ; Huang, X. 1 ; Liu, Q. 1 ; Li, Y. 1 ; Zhang, L. 1 ; Han, K. 1 ; Yang, J. 1 ; Liu, Y. 1 ; Xue, F. 1 ; Zhao, D. 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Coll Vet Med, 1 Tongwei Rd, Nanjing 210095, Jiangsu, Peoples R China
3.Jiangsu Univ, Inst Life Sci, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China
4.Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Jiangsu, Peoples R China
关键词： goose astrovirus; capsid protein; monoclonal antibody; epitope
期刊名称：POLISH JOURNAL OF VETERINARY SCIENCES （ 2022影响因子：0.8； 五年影响因子：0.9 ）
ISSN： 1505-1773
年卷期： 2022 年 25 卷 4 期
收录情况： SCI
摘要： Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope (33)QKVY(36) within Cap protein. Sequence alignment indicated that (33)QKVY(36) is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
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