Genome-Wide Characterization of the Isatis indigotica MADS-box Family and Role of IiSVP in Flowering
文献类型： 外文期刊
作者： Wei, L. 1 ; Sun, L. -Q 1 ; Zhang, C. -Y 1 ; Tang, X. -Q 1 ; Wang, F. Q. 2 ; Wang, K. -C 1 ; Yang, J. 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Peoples R China
关键词： Isatis indigotica; IiSVP; MADS-box genes; bioinformatics analysis; flowering
期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.4； 五年影响因子：1.6 ）
ISSN： 1021-4437
年卷期： 2023 年 70 卷 4 期
页码：
收录情况： SCI
摘要： MADS-box family genes play an important role in the development of plant reproductive growth, but there have been no genome-wide characterization reports on Isatis indigotica Fortune. In this study, eighty IiMADS genes were identified and clustered into 16 subgroups (3 type I and 13 type II) with reference to Arabidopsis MADS genes. Most IiMADS proteins in the same cluster had similar exon-intron and motif structures. Seventy-four IiMADS genes were randomly distributed among 7 chromosomes, and the remaining genes were contigs. The RNA-seq data showed that most IiMADS transcripts were highly enriched in roots, stems, flowers and fruits but rarely in leaves. Based on our phylogenetic and expression analyses, the IiSVP gene from I. indigotica was cloned and functionally characterized. qRT-PCR revealed that IiSVP is mainly expressed in stems, buds, flowers, and fruits. The IiSVP protein could interact with IiSOC1 and IiFLC, which was detected by BiFC and Y2H. The heterogeneous overexpression of IiSVP in Arabidopsis showed that it suppresses floral organ development, delays flowering and is significantly related to the expression of flowering genes such as FLC, SOC1 and GA20ox2. This study provided new information on the MADS family in I. indigotica and suggested that IiSVP is a key gene in suppressing flowering in I. indigotica.
- 相关文献
作者其他论文 更多>>
-
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
作者：Dai, G.;Huang, X.;Liu, Q.;Li, Y.;Zhang, L.;Han, K.;Yang, J.;Liu, Y.;Xue, F.;Zhao, D.;Dai, G.;Xue, F.;Zhao, D.;Zhao, D.;Dai, G.;Huang, X.;Liu, Q.;Li, Y.;Zhang, L.;Han, K.;Yang, J.;Liu, Y.;Zhao, D.
关键词：goose astrovirus; capsid protein; monoclonal antibody; epitope
-
Drought tolerance of transgenic rice overexpressing maize C-4-PEPC gene related to increased anthocyanin synthesis regulated by sucrose and calcium
作者：He, Y. F.;Li, X.;Yang, J.;Li, X.;Yang, J.;He, Y. F.;Xie, Y. F.
关键词：anthocyanin; calcium; C-4-pepc; drought response; rice; SnRK; sucrose; Oryza sativa
-
Characterization of Transcriptome Expression: The Response ofIsatis indigoticato Salt Stress
作者：Tang, X-Q;Tan, H-W;Shi, S-L;Wang, L-L;Wang, K-C;Wang, F-Q;Yang, J.
关键词：Isatis indigotica; de novo assembly; expression profile; RNA-seq; salt stress
-
Interaction of Water and Nitrogen Reveals Growth and Physiological Characteristics Responses of Isatis indigotica Fort. Seedlings
作者：Tang, X. Q.;Miao, Y. J.;Guan, J. L.;Zhang, K.;Wang, Y. R.;Yang, J.
关键词：Isatis indigotica; water and nitrogen interaction; growth; photosynthetic characteristics; antioxidant responses
-
Growth, secondary metabolism, and related gene expression in response to interactions of nitrogen and sulfur in Isatis indigotica
作者：Miao, Y. J.;Qu, R. J.;Sha, J. T.;Cao, Y. W.;Guan, J. L.;Xu, J.;Tang, X. Q.;Wang, F. Q.;Yang, J.
关键词：indigo; indirubin; photosynthetic rate; RT-qPCR; stomatal conductance; transpiration rate
-
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
作者：Xu, T.;Liu, X.;Liu, Q.;Han, K.;Liu, Y.;Yang, J.;Huang, X.;Zhao, D.;Bi, K.;Li, Y.;Xu, T.;Liu, X.;Sun, W.
关键词：goose-derived; duck-derived; chicken-derived; Tembusu virus; pathogenicity
-
Characterization of the Arabidopsis thaliana heme oxygenase 1 promoter in response to salinity, iron deficiency, and mercury exposure
作者：Wang, F. -Q.;Dai, C.;Zhang, Y. -H.;Shen, W. -B.;Wang, F. -Q.;Yang, J.;Wu, M. -Z.
关键词：beta-glucuronidase;MYB binding site;transgenic plants;5 '-untranslated region
