文献类型： 外文期刊

作者： Ming, Hong ¹ ; Cheng, Li-Jiao ² ; Ding, Chen-Long ³ ; Niu, Ming-Ming ² ; Zhao, Zhuo-Li ² ; Ji, Wei-Li ¹ ; Zhang, Ling- ¹ ;

作者机构： 1.Xinxiang Med Univ, Coll Life Sci & Technol, Synthet Biol Engn Lab Henan Prov, Xinxiang 453003, Henan, Peoples R China

2.Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Henan, Peoples R China

3.Jiangsu Acad Agr Sci, Suqian Inst Agr Sci, Suqian 223800, Peoples R China

关键词： Paracoccus luteus sp. nov.; grass carp; intestine; Polyphasic taxonomy

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 1 期

页码：

收录情况： SCI

摘要： A novel Gram-negative bacterium, designated CFH 10530(T), was isolated from the intestine of grass carp. The sample was collected from the aquaculture training base at the College of Fisheries, Henan Normal University, Xinxiang, PR China. Cells of strain CFH 10530(T) were coccoid, ovoid or short-rod-shaped, aerobic, non-spore-forming and non-motile. 16S rRNA gene sequence analysis demonstrated that strain CFH 10530(T) was closely related to Paracoccus endophyticus SYSUP0003(T) (97.7 % sequence similarity), Paracoccus halophilus HN-182(T) (96.5 %) and Paracoccus panacisoli DCY94T (96.1 %). The strain grew optimally at 25-28 degrees C, at pH 7.0 and with 0-2% (w/v) NaCl. Cells were positive for catalase and oxidase, nitrate was reduced and H2S was not produced. The isoprenoid quinone was Q-10. Major cellular fatty acids were summed feature 8, C-18:0 and C-18:03-OH. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and five unidentified polar lipids. The genome size was 3331 229 bp with a G+C content of 69.6 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH 10530(T) and the other species of the genus Paracoccus were found to be below the recommended levels for species delineation (ANIm <85, ANIb <80 and dDDH <24%). Based on its physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH 10530(T) is considered to represent a novel species for which the name Paracoccus luteus sp. nov., is proposed. The type strain is CFH 10530(T) (=KCTC 62919(T)=CGMCC 1.16597(T)).