文献类型： 外文期刊

作者： Zhao, Zhuo-Li ¹ ; Ming, Hong ² ; Ding, Chen-Long ³ ; Ji, Wei-Li ² ; Cheng, Li-Jiao ¹ ; Niu, Ming-Ming ¹ ; Zhang, Yan-M ¹ ;

作者机构： 1.Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Henan, Peoples R China

2.Xinxiang Med Univ, Coll Life Sci & Technol, Synthet Biol Engn Lab Henan Prov, Xinxiang 453003, Henan, Peoples R China

3.Jiangsu Acad Agr Sci, Suqian Inst Agr Sci, Suqian 223800, Peoples R China

关键词： Azospirillum thermophilum sp. nov.; hot spring; polyphasic taxonomy

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 1 期

页码：

收录情况： SCI

摘要： A novel Gram-staining negative, aerobic, motile by flagellum, rod-shaped bacterium, designated CFH 70021(T) was isolated from a hot spring soil sample collected from Tengchong, Yunnan province, PR China. Growth of CFH 70021(T) occurred at 15-50 degrees C (optimum 50 degrees C), pH 5.0-7.0 (optimum pH 7.0) and with 0-3.0% (w/v) NaCl (optimum 0 %, w/v). The genome of CFH 70021(T) consisted of four complete circular chromosomes and five plasmids, the genomic DNA G+C content was 69.3 mol%. Comparison of the 16S rRNA gene sequences indicated that CFH 70021(T) represented a member of the genus Azospirillum and showed close relationship with the type strains of Azospirillum agricola CC-HIH038(T) (97.8%), Azospirillum rugosum IMMIB AFH-6(T) (97.6%), Azospirillum doebereinerae GSF71(T) (97.6 %), Azospirillum thiophilum DSM 21654T (97.4%) and Azospirillum picis IMMIB TAR-3(T) (97.2%). The polar lipids of CFH 70021(T) contained diphosphatidylglycerol, phosphatidylmehtylethanolamine, phosphatidylglycerol, phosphatidylcholine, two aminolipids and an unidentified phospholipid. The predominant cellular fatty acids (>10%) included C(19:0)cyclo omega 8c (11.4 %), C-16:0 (27.6 %) and summed feature 8 (C (18:1)omega 7c/C-18:1 omega 6c, 40.9%). The major isoprenoid quinone was Q-10. On the basis of the low ANIb result (<78 %) and different phenotypic and chemotaxonomic characters, we conclude that strain CFH 70021(T) represents a novel member of the genus Azospirillum, for which the name Azospirillum thermophilum sp. nov. is proposed. The type strain is CFH 70021(T) (=KCTC 62259(T)= CCTCC AB2018121(T)).