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Cell Membrane-Interrupting Antimicrobial Peptides from Isatis indigotica Fortune Isolated by a Bacillus subtilis Expression System

文献类型： 外文期刊

作者： Wu, Jia 1 ; Abbas, Hafiz Muhammad Khalid 3 ; Li, Jiale 1 ; Yuan, Yuan 5 ; Liu, Yunjun 6 ; Wang, Guoying 6 ; Dong, Wube 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Dept Plant Pathol, Wuhan 430070, Hubei, Peoples R China

2.Huazhong Agr Univ, Key Lab Crop Dis Monitoring & Safety Control Hube, Wuhan 430070, Hubei, Peoples R China

3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Peoples R China

4.Guangdong Acad Agr Sci, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Peoples R China

5.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China

6.Chinese Acad Agr Sci, Inst Crop Sci, South St Zhongguancun 12, Beijing 100081, Peoples R China

关键词： antimicrobial peptide; plant resistance gene; membrane-interrupting peptides; gene expression system; Bacillus subtilis; Isatis indigotica

期刊名称：BIOMOLECULES （ 影响因子：4.879； 五年影响因子：5.362 ）

ISSN：

年卷期： 2020 年 10 卷 1 期

页码：

收录情况： SCI

摘要： The situation of drug resistance has become more complicated due to the scarcity of plant resistance genes, and overcoming this challenge is imperative. Isatis indigotica has been used for the treatment of wounds, viral infections, and inflammation for centuries. Antimicrobial peptides (AMPs) are found in all classes of life ranging from prokaryotes to eukaryotes. To identify AMPs, I. indigotica was explored using a novel, sensitive, and high-throughput Bacillus subtilis screening system. We found that IiR515 and IiR915 exhibited significant antimicrobial activities against a variety of bacterial (Xanthomonas oryzae, Ralstonia solanacearum, Clavibacter michiganensis, and C. fangii) and fungal (Phytophthora capsici and Botrytis cinerea) pathogens. Scanning electron microscope and cytometric analysis revealed the possible mechanism of these peptides, which was to target and disrupt the bacterial cell membrane. This model was also supported by membrane fluidity and electrical potential analyses. Hemolytic activity assays revealed that these peptides may act as a potential source for clinical medicine development. In conclusion, the plant-derived novel AMPs IiR515 and IiR915 are effective biocontrol agents and can be used as raw materials in the drug discovery field.

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