Evaluation and comparison of immune responses induced by two Mpox mRNA vaccine candidates in mice
文献类型： 外文期刊
作者： Yang, Xidan 1 ; Hu, Congxia 1 ; Yang, Xuetao 1 ; Yang, Xiu 1 ; Hu, Xing 3 ; Wang, Xingyun 3 ; Liu, Cong 2 ; Yuan, Yuan 1 ; Du, Shouwen 4 ; Wang, Peng George 2 ; Lin, Jihui 1 ;
作者机构： 1.Southwest Med Univ, Sch Nursing, Luzhou 646000, Sichuan, Peoples R China
2.Pengbo Biotechnol Co Ltd, Shenzhen 518000, Peoples R China
3.Southern Univ Sci & Technol, Sch Med, Dept Pharmacol, Shenzhen, Peoples R China
4.Guangdong Acad Agr Sci, Key Lab Livestock Dis Prevent Guangdong Prov, Key Lab Prevent & Control Avian Influenza & Other, Inst Anim Hlth,Minist Agr & Rural Affairs, Guangzhou 510000, Peoples R China
关键词： antigen proteins; immune response; Mpox virus; mRNA vaccine; one-component and multicomponent
期刊名称：JOURNAL OF MEDICAL VIROLOGY （ 影响因子：12.7； 五年影响因子：8.5 ）
ISSN： 0146-6615
年卷期： 2023 年 95 卷 10 期
页码：
收录情况： SCI
摘要： The epidemic of Mpox virus (MPXV) from May 2022 was once declared as a Public Health Emergency of International Concern by the World Health Organization. Vaccines play an important role in prevention of infectious diseases, and mRNA vaccine technology was proved to be a safe and effective platform with successful application in defense of coronavirus disease 2019. In this study, based on A29L, M1R, A35R, and B6R of MPXV, we developed two MPXV mRNA vaccine candidates, designated as MPXfus and MPXmix. The MPXfus was one-component, in which these four antigen proteins were linked in tandem by flexible linker and encoded by an individual mRNA as a fusion protein. The MPXmix was multicomponent containing four mRNA, and each mRNA encoded one antigen protein respectively. Mice were immunized with equal quality of MPXfus or MPXmix, delivered by lipid nanoparticles for evaluation and comparison of the immune responses induced by these two MPXV vaccine candidates. Results of immune response analyses indicated that both MPXfus and MPXmix could elicit high-level of antigen-specific antibodies and robust cellular immune response in mice. Moreover, results of virus neutralization assays suggested that sera from MPXfus- or MPXmix-immunized mice possessed high neutralizing activities against vaccinia virus. In addition, titers of antigen-specific antibody, levels of cellular immune response, and activities of neutralizing antibody against vaccinia virus induced by MPXfus and MPXmix presented no significant difference. In summary, this study provides valuable insights for further clinical development of one-component and multicomponent mRNA vaccine candidates for the prevention of MPXV and other orthomyxoviruses.
- 相关文献
作者其他论文 更多>>
-
N-glycosylation on hemagglutinin head reveals inter-branch antigenic variability of avian influenza virus H5-subtypes
作者：Shi, Keyi;Feng, Saixiang;Zhao, Li;Chen, Junhong;Song, Wei;Jia, Yusheng;Qu, Xiaoyun;Jia, Weixin;Liao, Ming;Liu, Zhicheng;Du, Shouwen;Liao, Ming;Liao, Ming;Du, Shouwen
关键词：H5-subtype AIV; N-glycosylation; Antigenicity of hemagglutinin
-
IFITM3 inhibits severe fever with thrombocytopenia syndrome virus entry and interacts with viral Gc protein
作者：Du, Shouwen;Liu, Quan;Liao, Ming;Du, Shouwen;Zhou, Boping;Wang, Jigang;Wang, Yuhang;Wang, Jiamin;Ma, Yidan;Xu, Wang;Shi, Xiaoshuang;Li, Letian;Hao, Pengfei;Jin, Ningyi;Li, Chang;Wong, Yin K.;Wang, Jigang;Hu, Lifen;Liu, Wei
关键词：antiviral activity; Gc subunit; interferon-induced transmembrane protein 3; SFTSV; virus-IFITM3 interaction
-
Cryo-EM structure of severe fever with thrombocytopenia syndrome virus
作者：Du, Shouwen;Qu, Xiaoyun;Sun, Minhua;Wei, Zhengkai;Liu, Quan;Liao, Ming;Du, Shouwen;Wang, Yuhang;Guan, Yudong;Wang, Jigang;Liu, Fengting;Du, Shouwen;Wang, Guoqing;Liu, Quan;Li, Chang;Du, Shouwen;Wang, Guoqing;Liu, Quan;Li, Chang;Peng, Ruchao;Xu, Wang;Wang, Jiamin;Li, Letian;Tian, Mingyao;Deng, Lingcong;Shi, Xiaoshuang;Ma, Yidan;Jin, Ningyi;Li, Chang;Qu, Xiaoyun;Liao, Ming;Li, Hao;Liu, Wei;Qi, Jianxun;Qi, Jianxun;Liu, Quan
关键词：
-
The "LLQY" Motif on SARS-CoV-2 Spike Protein Affects S Incorporation into Virus Particles
作者：Du, Shouwen;Wang, Yuhang;Li, Tiyuan;Wu, Shipin;Zhou, Boping;Liu, Quan;Liao, Ming;Xu, Wang;Li, Letian;Hao, Pengfei;Tian, Mingyao;Jin, Ningyi;Li, Chang;Wang, Maopeng;Bai, Jieying;Qu, Xiaoyun;Liao, Ming
关键词：SARS-CoV-2; spike protein; LLQY domain; protein synthesis and cleavage; virus assembly
-
Identification and Fine Mapping of the Recessive Gene BK-5, Which Affects Cell Wall Biosynthesis and Plant Brittleness in Maize
作者：Li, Qigui;Nie, Shujun;Lin, Haijian;Pan, Guangtang;Li, Gaoke;Du, Jiyuan;Ren, Ruchang;Liu, Boyan;Gao, Xiaolong;Liu, Tianjian;Zhang, Zhiming;Zhao, Xiangyu;Li, Xinzheng;Nie, Yongxin;Ding, Haiping;Yang, Xiu;Wang, Baichen
关键词：maize; brittle stalk; cell wall; cellulose synthase catalytic subunit (CESA)
-
Identification of coronaviruses in farmed wild animals reveals their evolutionary origins in Guangdong, southern China
作者：Wang, Xiaohu;Ren, Zhaowen;Wang, Lu;Chen, Jing;Zhang, Pian;Chen, Xiaofan;Lin, Xuhui;Qi, Nanshan;Luo, Shengjun;Xiang, Rong;Zhang, Jianfeng;Wang, Gang;Sun, Min-Hua;Huang, Yuan;Huang, Zhong;Xiang, Hua;Sun, Mingfei;Liu, Quan;Liao, Ming;Wang, Xiaohu;Ren, Zhaowen;Wang, Lu;Chen, Jing;Zhang, Pian;Chen, Xiaofan;Lin, Xuhui;Qi, Nanshan;Luo, Shengjun;Xiang, Rong;Zhang, Jianfeng;Wang, Gang;Sun, Min-Hua;Huang, Yuan;Huang, Zhong;Xiang, Hua;Sun, Mingfei;Liu, Quan;Liao, Ming;Ren, Zhaowen;Wang, Lu;Zhang, Pian;Chen, Xiaofan;Yuan, Ziguo;Liao, Ming;Chen, Jin-Ping;Li, Linmiao;Hua, Yan;Zou, Jiejian;Hou, Fanghui;Du, Shouwen;Liu, Quan
关键词：coronavirus; wild animals; Rhizomys sinensis; Phasianus colchicus; Paguma larvata; southern China
-
The global succinylation of SARS-CoV-2-infected host cells reveals drug targets
作者：Liu, Quan;Sun, Minhua;Liao, Ming;Liu, Quan;Sui, Liyan;Zhao, Yinghua;Zhao, Yicheng;Liu, Quan;Zhang, He;Li, Letian;Xu, Wang;Hao, Pengfei;Jiang, Yuhang;Chen, Jing;Tian, Mingyao;Li, Xiao;Lu, Huijun;Zhuang, Xinyu;Jin, Ningyi;Li, Chang;Liu, Quan;Wei, Zhengkai;Wang, Heming;Song, Guangqi;Du, Shouwen;Qu, Xiaoyun;Liao, Ming;Guo, Xuerui;Wang, Xingye;Song, Wu;Song, Guangqi;Hou, Zhijun;Wang, Guoqing;Liao, Ming
关键词：SARS-CoV-2; succinylproteomics; antiviral; SIRT5; NSP14
