文献类型： 外文期刊

作者： Du, Shouwen ¹ ; Peng, Ruchao ⁵ ; Xu, Wang ⁶ ; Qu, Xiaoyun ¹ ; Wang, Yuhang ² ; Wang, Jiamin ⁶ ; Li, Letian ⁶ ; Tian, Mingyao ⁶ ; Guan, Yudong ² ; Wang, Jigang ² ; Wang, Guoqing ³ ; Li, Hao ⁸ ; Deng, Lingcong ⁶ ; Shi, Xiaoshuang ⁶ ; Ma, Yidan ⁶ ; Liu, Fengting ² ; Sun, Minhua ¹ ; Wei, Zhengkai ¹ ; Jin, Ningyi ⁶ ; Liu, Wei ⁸ ; Qi, Jianxun ⁹ ; Liu, Quan ¹ ; Liao, Ming ¹ ; Li, Chang ³ ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Key Lab Livestock Dis Prevent Guangdong Prov, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou, Peoples R China

2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Shenzhen, Peoples R China

3.Jilin Univ, Hosp 1, Dept Infect Dis, Changchun, Peoples R China

4.Jilin Univ, Hosp 1, Ctr Infect Dis & Pathogen Biol, Key Lab Organ Regenerat & Transplantat,Minist Educ, Changchun, Peoples R China

5.Univ Penn, Perelman Sch Med, Dept Biochem & Biophys, Philadelphia, PA USA

6.Chinese Acad Agr Sci, Changchun Vet Res Inst, Chinese Acad Med Sci, Res Unit Key Technol Prevent & Control Virus Zoono, Changchun, Peoples R China

7.South China Agr Univ, Key Lab Zoonosis Minist Agr & Rural Affairs, Guangzhou, Peoples R China

8.Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China

9.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China

10.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, Peoples R China

11.Foshan Univ, Guangdong Acad Sci, Inst Zool, Guangdong Key Lab Anim Conservat & Resource Utiliz, Foshan, Peoples R China

期刊名称：NATURE COMMUNICATIONS （ 影响因子：16.6； 五年影响因子：17.0 ）

ISSN：

年卷期： 2023 年 14 卷 1 期

页码：

收录情况： SCI

摘要： The severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne human-infecting bunyavirus, which utilizes two envelope glycoproteins, Gn and Gc, to enter host cells. However, the structure and organization of these glycoproteins on virion surface are not yet known. Here we describe the structure of SFTSV determined by single particle reconstruction, which allows mechanistic insights into bunyavirus assembly at near-atomic resolution. The SFTSV Gn and Gc proteins exist as heterodimers and further assemble into pentameric and hexameric peplomers, shielding the Gc fusion loops by both intra- and inter-heterodimer interactions. Individual peplomers are associated mainly through the ectodomains, in which the highly conserved glycans on N914 of Gc play a crucial role. This elaborate assembly stabilizes Gc in the metastable prefusion conformation and creates some cryptic epitopes that are only accessible in the intermediate states during virus entry. These findings provide an important basis for developing vaccines and therapeutic drugs. The severe fever with thrombocytopenia syndrome virus (SFTSV, officially named Dabie bandavirus) is a tick-borne human-infecting bunyavirus. Here, the authors report the cryo-EM structure of SFTSV virion, and discuss the mechanisms of viral assembly, membrane fusion and antibody neutralization.