文献类型： 外文期刊
作者： Zhao, Shanshan 1 ; Du, Shouwen 3 ; Guan, Yudong 1 ; Schlueter, Hartmut 2 ;
作者机构： 1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Crit Care Med,Guangdong Prov Clin Res Ctr Ger, Shenzhen 518020, Guangdong, Peoples R China
2.Univ Med Ctr Hamburg Eppendorf, Ctr Diagnost, Sect Mass Spectrometry & Prote, D-20246 Hamburg, Germany
3.Guangdong Acad Agr Sci, Key Lab prevent & control Avian Influenza & Other, Minist Agr & Rural Affairs, Guangzhou 510640, Guangdong, Peoples R China
4.Guangdong Acad Agr Sci, Inst Anim Hlth, Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Guangdong, Peoples R China
5.Henan Univ, Sch Stomatol, Kaifeng 475000, Henan, Peoples R China
6.Zhongzhou Lab, Zhengzhou 450046, Henan, Peoples R China
关键词： N-glycomics; N-sialoglycans; nMatch; Mass spectrometry
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：3.3； 五年影响因子：3.1 ）
ISSN： 0273-2289
年卷期： 2025 年
页码：
收录情况： SCI
摘要： The structural instability and electronegativity of sialic acids make N-sialoglycan profiling challenging. We compared commonly used preparation strategies, including native, acetohydrazide-based derivatization, and permethylation, in N-glycomics with nMatch, an R-based bioinformatics tool that ensures full coverage of the detected N-glycans using mass spectrometry. We demonstrated that permethylation leads to efficient detection of not only N-sialoglycans but also other N-glycans and, therefore, provides a valuable reference for the selection of the appropriate N-glycan preparation strategy in future studies.
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