文献类型： 外文期刊

作者： Lin, Qijie ¹ ; Gou, Hongchao ² ; Qu, Xiaoyun ¹ ; Jia, Kaiyuan ³ ; Deng, Yuhui ¹ ; Li, Dan ⁴ ; Cai, Qianyi ¹ ; Liang, Yucen ¹ ; Xu, Xiaozhen ¹ ; Li, Yanbin ⁵ ; Lin, Jianhan ³ ; Li, Letian ⁶ ; Jiang, Yuhang ⁶ ; Du, Shouwen ² ; Deng, Lingcong ⁶ ; Yan, Bailing ⁴ ; Liu, Ruidong ¹ ; Li, Chang ⁶ ; Zhang, Jianmin ¹ ; Liao, Ming ¹ ;

作者机构： 1.South China Agr Univ, Natl & Reg Joint Engn Lab Medicament Zoonoses Prev, Coll Vet Med,Minist Agr,Anim Infect Dis Lab, Key Lab Zoonoses,Key Lab Zoonoses Prevent & Contro, Guangzhou 510642, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangdong Prov Key Lab Livestock Dis Prevent, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou 510640, Peoples R China

3.China Agr Univ, Key Lab Agr Informat Acquisit Technol, Minist Agr & Rural Affairs, Beijing 100083, Peoples R China

4.First Hosp Jilin Univ, Ctr Infect Dis & Pathogen Biol, Key Lab Organ Regenerat & Transplantat, Dept Resp Med,Minist Educ,State Key Lab Zoonot Dis, Changchun 130021, Peoples R China

5.Univ Arkansas, Ctr Excellence Poultry Sci, Dept Biol & Agr Engn, Fayetteville, AR 72701 USA

6.Chinese Acad Med Sci, Changchun Vet Res Inst, Res Unit Key Technol Prevent & Control Virus Zoono, Changchun 130062, Peoples R China

7.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China

关键词： nucleic acid amplification; SARS-CoV-2 variants of concern; SNP detection; ssLAMP

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年 12 卷 26 期

页码：

收录情况： SCI

摘要： Rapid on-site typing methods for SARS-CoV-2 variants of concern are crucial for its effective surveillance and control. Herein, a smart single-loop-mediated isothermal amplification (ssLAMP) method with the absence of an inner primer but the addition of a swarm primer for differentiation of SARS-CoV-2 Omicron variants is developed. This unique primer design strategy offers greater flexibility in introducing single nucleotide polymorphism (SNP) identification probes and enables multiple detection assays for SARS-CoV-2 Omicron variants including BA.1, BA.2, BA.3, BA.4, and BA.5. A 3D-printed portable dual fluorescence visualization device and smartphone app are developed to enable point-of-care testing. This assay is rapid (within 90 min), highly sensitive (100 copies/reaction), and specific (identification of SNP) for SARA-CoV-2 Omicron variants. The ssLAMP method identifies five BA.5-positive samples among 97 nasopharyngeal swab samples from the clinic, with a 100% concordance rate with Sanger sequencing. The ssLAMP assay system is expected to be utilized for on-site, highly specific, and rapid visualization detection of SARS-CoV-2 and its variants, with great application potential in pathogen genotyping, early cancer screening, and other areas of SNP mutation detection.