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Cytological atlas at meiosis reveals insights into pollen fertility in synthetic Brassica allotriploids between allotetraploid B. carinata and diploid B. rapa

文献类型： 外文期刊

作者： Yang, Yan 1 ; Yan, Ge 2 ; Li, Zishuang 1 ; Yuan, Jiachen 1 ; Wei, Xiaochun 3 ; Wei, Fang 1 ; Tian, Baoming 2 ; Xie, Zheng 1 ;

作者机构： 1.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China

2.Zhengzhou Univ, Sch Agr Sci, Zhengzhou 450001, Henan, Peoples R China

3.Henan Acad Agr Sci, Inst Hort Res, Zhengzhou 450002, Henan, Peoples R China

关键词： Interspecific hybridization; Meiosis; Pollen fertility; Brassica allotriploids; Polyploidy; Brassica carinata; Brassica rapa

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 148 卷

页码：

收录情况： SCI

摘要： The formation of allopolyploid crops basically depends on the successful interspecific hybridization and poly-ploidization, which generally involves in a combination of distinct but related genomes from independent species. But cytological analysis of these initially synthesized allohaploids immediately after genome merging is poorly explored in regards to anther and pollen development to date. In this study, Brassica trigenomic allohaploids (ABC) were produced to investigate the immediate effects of the genome combinations on pollen fertility during anther development via crosses between natural allotetraploid B. carinata (BBCC) and diploid B. rapa (AA). The results showed that in the synthetic Brassica allotriploids (ABC), the anther development was completely disrupted, and the pollen grains were mostly inviable with varied genetic complements. In addition, the meiosis course was aberrantly altered and eccentric chromosomal configurations including multivalent, bridges and lags occurred frequently during metaphase I to anaphase II. Genomic in situ hybridization (GISH) further revealed that B genome of homoeology was frequently apt to interact with A and C genomes, and cytoskeletal organizations was improperly distributed during meiosis in these synthetic Brassica allotriploids. Furthermore, we also confirmed that the expression of typical meiosis-related genes was obviously repressed during anther development in these Brassica allotriploids. Taken together, our results provide a detailed cytology for insights into pollen development in the synthetic allotriploid hybrids, which are conventionally considered as a useful genetic resource for polyploid Brassica breeding.

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