Combined Linkage Mapping and BSA to Identify QTL and Candidate Genes for Plant Height and the Number of Nodes on the Main Stem in Soybean
文献类型： 外文期刊
作者： Li, Ruichao 1 ; Jiang, Hongwei 2 ; Zhang, Zhanguo 1 ; Zhao, Yuanyuan 1 ; Xie, Jianguo 1 ; Wang, Qiao 1 ; Zheng, Haiyan 1 ;
作者机构： 1.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China
2.Jilin Acad Agr Sci, Soybean Res Inst, Changchun 130033, Peoples R China
关键词： PH and NNMS; BSA; QTL; haplotype analysis; soybean
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2020 年 21 卷 1 期
页码：
收录情况： SCI
摘要： Soybean is one of the most important food and oil crops in the world. Plant height (PH) and the number of nodes on the main stem (NNMS) are quantitative traits closely related to soybean yield. In this study, we used 208 chromosome segment substitution lines (CSSL) populations constructed using "SN14" and "ZYD00006" for quantitative trait locus (QTL) mapping of PH and NNMS. Combined with bulked segregant analysis (BSA) by extreme materials, 8 consistent QTLs were identified. According to the gene annotation of the QTL interval, a total of 335 genes were obtained. Five of which were associated with PH and NNMS, potentially representing candidate genes. RT-qPCR of these 5 candidate genes revealed two genes with differential relative expression levels in the stems of different materials. Haplotype analysis showed that different single nucleotide polymorphisms (SNPs) between the excellent haplotypes in Glyma.04G251900 and Glyma.16G156700 may be the cause of changes in these traits. These results provide the basis for research on candidate genes and marker-assisted selection (MAS) in soybean breeding.
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