Development of SNP markers for yellowtail kingfish (Seriola lalandi) by 2b-RAD simplified genome sequencing
文献类型： 外文期刊
作者： Cui, Aijun 1 ; Wang, Bin 1 ; Jiang, Yan 1 ; Liu, Xuezhou 1 ; Xu, Yongjiang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries & Food Prod Proc, Qingdao 266071, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词： Seriola lalandi; 2b-RAD sequencing; SNP markers; Population genetics
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）
ISSN： 1877-7252
年卷期：
页码：
收录情况： SCI
摘要： Yellowtail kingfish, Seriola lalandi, is a globally distributing pelagic marine fish species. In recent years, the wild population has suffered drastic decline due to overfishing and environmental deterioration. Thus, the sustainable utilization and conservation management for this species should be implemented urgently. In the present study, we developed SNP markers using 2b-RAD sequencing method from 33 individuals of yellowtail kingfish. Wherein, 100 high-quality SNP markers were obtained based on high throughput sequencing and stringent SNP filter process. The minor allele frequency of these SNPs ranged from 0.0126 to 0.4957. The observed heterozygosity and expected heterozygosity ranged from 0 to 0.9914 and 0.0249 to 0.5000, respectively. The inbreeding coefficient varied from - 0.9828 to 0.7882. Among these SNPs, 10 loci deviated significantly from Hardy-Weinberg equilibrium after Bonferroni correction. These novel SNP markers will serve as valuable tools for population genetics evaluation and natural resources conservation of yellowtail kingfish.
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