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Isolation of urease-producing bacteria and their effects on reducing Cd and Pb accumulation in lettuce (Lactuca sativa L.)

文献类型： 外文期刊

作者： Wang, Tiejun 1 ; Wang, Shilin 1 ; Tang, Xingchun 1 ; Fan, Xianpeng 2 ; Yang, Sheng 1 ; Yao, Lunguang 3 ; Li, Yadong 1 ; H 1 ;

作者机构： 1.Hubei Univ, State Key Lab Biocatalysis & Enzyme Engn, Sch Life Sci, Wuhan 430062, Peoples R China

2.Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan 430062, Peoples R China

3.Nanyang Normal Univ, Collaborat Innovat Water Secur Water Source Reg M, South To North Divers Project Henan Prov, Coll Agr Engn, Nanyang 473061, Peoples R China

关键词： Urease; Urease-producing bacteria; Extracellular adsorption; Lettuce; Heavy metal immobilization; Soluble protein

期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）

ISSN： 0944-1344

年卷期： 2020 年 27 卷 8 期

页码：

收录情况： SCI

摘要： Excess Cd and Pb in agricultural soils enter the food chain and adversely affect all organisms. Therefore, it is important to find an eco-friendly way to reduce heavy metal accumulation in vegetables. We used urea agar plates to isolate urease-producing bacteria from the rhizosphere soil of lettuce in Cd- and Pb-contaminated farmland and investigated their ability to produce urease and immobilize heavy metals. The effects of these strains on the biomass, quality, and Cd and Pb accumulation of lettuce were also studied. The results showed that two urease-producing bacteria, Enterobacter bugandensis TJ6 and Bacillus megaterium HD8, were screened from the rhizosphere soil of lettuce. They had a high ability to produce urease (44.5 mS cm(-1) min(-1) OD600-1 and 54.2 mS cm(-1) min(-1) OD600-1, respectively) and IAA (303 mg L-1 and 387 mg L-1, respectively). Compared with the control, inoculation with strains TJ6 and HD8 reduced the Cd (75.3-85.8%) and Pb (74.8-87.2%) concentrations and increased the pH (from 6.92 to 8.13-8.53) in solution. A hydroponic experiment showed that the two strains increased the biomass (31.3-55.2%), improved the quality (28.6-52.6% for the soluble protein content and 34.8-88.4% for the vitamin C (Vc) content), and reduced the Cd (25.6-68.9%) and Pb (48.7-78.8%) contents of lettuce shoots (edible tissue). In addition, strain HD8 had a greater ability than strain TJ6 to reduce lettuce Cd and Pb uptake and water-soluble Cd and Pb levels in solution. These data show that the urease-producing bacteria protect lettuce against Cd and Pb toxicity by extracellular adsorption, Cd and Pb immobilization, and increased pH. The effects of heavy metal immobilization by the two strains can guarantee vegetable safety in situ for the bioremediation of heavy metal-polluted farmland.

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