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Membrane IgM(+) plasma cells in grass carp (Ctenopharyngodon idella): Insights into the conserved evolution of IgM(+) plasma cells in vertebrates

文献类型： 外文期刊

作者： Cui, Zheng-Wei 1 ; Zhang, Xiang-Yang 4 ; Wu, Chang-Song 1 ; Zhang, Yong-An 1 ; Zhou, Yang 1 ; Zhang, Xu-Jie 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

3.Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Minist Agr China, River Fisheries Res Inst, Guangzhou 510380, Peoples R China

4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430070, Peoples R China

关键词： IgM(+) B cell; Plasma cell; CD4OL; IL-21; Grass carp

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2020 年 106 卷

页码：

收录情况： SCI

摘要： Teleost fish are the most primitive bony vertebrates that contain B cells; thus, comparative analysis of teleost naive/mature B cells and plasma cells can provide helpful evidence for understanding the evolution paradigms of these two B-cell subpopulations in vertebrates. In this study, we developed monoclonal antibody against grass carp IgM and identified two different IgM(+) cell subsets: IgM(+) lymphocytes (Lym), resembling naive/mature B cells, and IgM(+) myeloid cells (Mye), resembling plasma cells. Like plasma cells in mammals, the size of IgM(+) Mye is significantly larger than that of IgM(+) Lym, as revealed by flow cytometric analysis and transmission electron microscopy. The IgM(+) Mye were further verified as plasma cells because they showed gene expression patterns similar with those of human plasma cells and a great capacity to secrete IgM. Like mammalian IgM(+) and IgA(+) plasma cells, not IgG(+) plasma cells, grass carp IgM(+) Mye also expressed membrane immunoglobulins, a feature conserved in IgM(+) plasma cells in vertebrates. Furthermore, recombinant CD4OL or IL-21 alone could induce the plasma cell generation and IgM secretion, while the combination of CD4OL and IL-21 had greater effect on IgM secretion, but not on plasma cell generation. This study fills an important gap in the knowledge of plasma cells in teleost fish and provides critical insights into the conserved evolution of IgM(+) plasma cells in vertebrates.

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