Gold nanoparticles enhance immune responses in mice against recombinant classical swine fever virus E2 protein
文献类型： 外文期刊
作者： Li, Yafei 1 ; Jin, Qianyue 1 ; Ding, Peiyang 4 ; Zhou, Wen 1 ; Chai, Yongxiao 1 ; Li, Xufeng 1 ; Wang, Yao 1 ; Zhang, Gai 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
4.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Peoples R China
5.Henan Agr Univ, Coll Anim Husb & Vet Sci, Zhengzhou 450002, Peoples R China
关键词： Gold nanoparticles; Classical swine fever virus; E2 protein; Carrier; Subunit vaccine
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
ISSN： 0141-5492
年卷期：
页码：
收录情况： SCI
摘要： Objective To create a novel subunit vaccine that used AuNPs as carriers to enhance immune responses in mice against recombinant classical swine fever virus E2 protein (CSFV E2). Results Gold nanoparticles (AuNPs) were successfully coupled to the E2 protein and formed stable particle complexes called E2 conjugated AuNPs (E2-AuNPs). In vitro studies have shown that the E2-AuNPs complex has the same immunogenicity as the E2 protein, and AuNPs can promote the phagocytosis of the E2 protein by antigen-presenting cells (APCs). In vivo results of BALB/c mice showed that the antibody levels, lymphocyte proliferation index, IFN-gamma and IL-10 cytokines induced by E2-AuNPs were relatively higher than those of E2 or AuNPs group. Conclusions This finding demonstrated the potential of using AuNPs as a carrier to enhance the body's immune response for developing CSFV subunit vaccines. This model also contributes to the development of other flavivirus subunit vaccines, such as hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV).
- 相关文献
作者其他论文 更多>>
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization
作者：Li, Minghui;Sun, Xueke;Wang, Siqiao;Wang, Yanan;Wang, Yue;Zhang, Gaiping;Li, Minghui;Sun, Xueke;Chen, Yilan;Wang, Siqiao;Li, Qin;Wang, Yanan;Wang, Yue;Li, Ruiqi;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：PEDV; S1 protein; Nanoparticle; Subunit vaccine; Mucosal immunity
-
Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway
作者：Kong, Zhengjie;Zhang, Yifeng;Guan, Kaifeng;Yao, Wanzi;Kang, Yu;Lu, Xinyi;Zhang, Gaiping;Chen, Xing;Gong, Lele;Wang, Lele;Zhang, Yuhang;Du, Yongkun;Sun, Aijun;Zhuang, Guoqing;Wan, Bo;Zhang, Gaiping;Zhao, Jianguo;Zhang, Gaiping;Zhang, Gaiping
关键词：pseudorabies virus; cGAS-STING; tegument protein US2; TRIM21; immune invasion
-
IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner
作者：Shi, Xibao;Wei, Menglu;Feng, Yuwen;Yang, Yuanhao;Zhang, Xiaozhuan;Chen, Hao;Xing, Yuqi;Wang, Keqi;Wang, Wensheng;Wang, Li;Zhang, Gaiping;Wang, Aiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Shi, Xibao;Zhang, Gaiping
关键词：IFN-gamma-inducible protein 16; interferon gene-stimulating protein; retinoic acid-inducible gene I; type I interferon
-
Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration
作者：Liu, Xin-Xin;Wang, Yao;Lu, Yun-Feng;Dong, Zi-Xing;Yue, Chao;Li, Dan-Dan;Yao, Lun-Guang;Tang, Cun-Duo;Liu, Xin-Xin;Wang, Yao;Lu, Yun-Feng;Dong, Zi-Xing;Yue, Chao;Li, Dan-Dan;Yao, Lun-Guang;Tang, Cun-Duo;Zhang, Jian-Hui;Tang, Cun-Duo;Huang, Xian-Qing;Tang, Cun-Duo;Zhang, Si-Pu
关键词：2,5-dimethylpyrazine; Metabolic engineering; Microbial cell factories; Aminoacetone oxidase; Threonine transporter; Whole-cell catalysis
-
A universal design of restructured dimer antigens: Development of a superior vaccine against the paramyxovirus in transgenic rice
作者：Ma, Fanshu;Zhang, Longxian;Zhang, Erqin;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Xu, Qianru;Yang, Daichang;Li, Qingmei;Guo, Junqing;Li, Rui;Wang, Li;Wang, Yanan;Zhao, Xiangyue;Chai, Shujun;Yin, Heng;Li, Kunpeng;Rao, Zihe;Rao, Zihe;Rao, Zihe
关键词：structural vaccine; transgenic rice; BCR; paramyxovirus
-
Double-layered N-S1 protein nanoparticle immunization elicits robust cellular immune and broad antibody responses against SARS-CoV-2
作者：Li, Ruiqi;Chang, Zejie;Cheng, Anchun;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Chang, Zejie;Wang, Yanan;Li, Minghui;Chen, Yilan;Fan, Lu;Wang, Siqiao;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Chang, Zejie;Wang, Yanan;Li, Minghui;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Liu, Hongliang;Ding, Peiyang;Zhang, Gaiping
关键词：COVID-19; Coronavirus; SARS-CoV-2; Nanoparticle; Subunit vaccine; Variants
-
Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication
作者：Zhao, Shuang-shuang;Qian, Qisheng;Zhang, Gaiping;Zhao, Shuang-shuang;Qian, Qisheng;Chen, Xin-xin;Lu, Qingxia;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Zhang, Gaiping
关键词：autophagy; GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1
