Transcriptome analysis and weighted gene co-expression network reveals potential genes responses to heat stress in turbot Scophthalmus maximus
文献类型： 外文期刊
作者： Huang, Zhihui 1 ; Ma, Aijun 1 ; Yang, Shuangshuang 1 ; Liu, Xiaofei 1 ; Zhao, Tingting 1 ; Zhang, Jinsheng 1 ; Wang, X 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Shandong Key Lab Marine Fisheries Biotechnol & Ge, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Yantai Tianyuan Aquat Ltd Corp, Yantai 264006, Peoples R China
关键词： Heat stress; RNA-seq; WGCNA; Gene expression; Hub genes; Scophthalmus maximus
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.674； 五年影响因子：2.941 ）
ISSN： 1744-117X
年卷期： 2020 年 33 卷
页码：
收录情况： SCI
摘要： Turbot (Scophthalmus maximus) is an economically important marine fish cultured in China. In this study, we performed transcriptome gene expression profiling of kidney tissue in turbot exposed to heat stress (20, 23, 25 and 28 degrees C); control fish were maintained at 14 degrees C. We investigated gene relationships based on weighted gene co-expression network analysis (WGCNA). Accordingly, enrichment analyses of GO terms and KEGG pathways showed that several pathways (e.g., fat metabolism, cell apoptosis, immune system, and insulin signaling) may be involved in the response of turbot to heat stress. Moreover, via WGCNA, we identified 19 modules: the dark grey module was mainly enriched in pathways associated with fat metabolism and the FOXO and Jak-STAT signaling pathways. The ivory module was significantly enriched in the P53 signaling pathway. Furthermore, the key hub genes CBP, AKT3, CCND2, PIK3r2, SCOS3, mdm2, eye-B, and p48 were enriched in the FOXO, Jak-STAT and P53 signaling pathways. This is the first study reporting co-expression patterns of a gene network after heat stress in marine fish. Our results may contribute to our understanding of the underlying molecular mechanism of thermal tolerance.
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