文献类型： 外文期刊
作者： Ma, Rui 1 ; Yu, Zhijing 1 ; Cai, Qinan 1 ; Li, Haiyun 1 ; Dong, Yingshan 1 ; Oksman-Caldentey, Kirsi-Marja 2 ; Rischer 1 ;
作者机构： 1.Jilin Acad Agr Sci, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China
2.VTT Tech Res Ctr Finland Ltd, POB 1000, FI-02044 Espoo, Finland
关键词： Veratrum dahuricum; cyclopamine; Agrobacterium tumefaciens; transformation
期刊名称：PLANTS-BASEL （ 影响因子：3.935； ）
ISSN：
年卷期： 2020 年 9 卷 2 期
页码：
收录情况： SCI
摘要： Veratrum dahuricum L. (Liliaceae), a monocotyledonous species distributed throughout the Changbai mountains of Northeast China, is pharmaceutically important, due to the capacity to produce the anticancer drug cyclopamine. An efficient transformation system of Veratrum dahuricum mediated with Agrobacterium tumefaciens is presented. Murashige and Skoog (MS) medium containing 8 mg/L picloram was used to induce embryogenic calli from immature embryos with 56% efficiency. A. tumefaciens LBA4404 carrying the bar gene driven by the cauliflower mosaic virus 35S promoter was employed for embryogenic callus inoculation. A. tumefaciens cell density OD660 = 0.8 for inoculation, half an hour infection period, and three days of co-culture duration were found to be optimal for callus transformation. Phosphinothricin (PPT, 16 mg/L) was used as the selectable agent, and a transformation efficiency of 15% (transgenic plants/100 infected calli) was obtained. The transgenic nature of the regenerated plants was confirmed by PCR and Southern blot analysis, and expression of the bar gene was detected by RT-PCR and Quick PAT/bar strips. The steroid alkaloids cyclopamine, jervine, and veratramine were detected in transgenic plants, in non-transformed and control plants collected from natural sites. The transformation system constitutes a prerequisite for the production of the pharmaceutically important anticancer drug cyclopamine by metabolic engineering of Veratrum.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification and Comprehensive Analysis of the PPO Gene Family in Glycine max and Glycine soja
作者：Song, Ziye;Dong, Yingshan;Song, Ziye;Wang, Bo;Liu, Jia;Liu, Nianxi;Yi, Zhigang;Li, Zhi;Dong, Zhimin;Zhang, Chunbao;Dong, Yingshan;Li, Yuqiu
关键词：
Glycine max ;Glycine soja ;PPO ; gene family -
Functional Verification of the Soybean Pseudo-Response Factor GmPRR7b and Regulation of Its Rhythmic Expression
作者：Song, Ziye;Liu, Jia;Qian, Xueyan;Wang, Bo;Liu, Nianxi;Yi, Zhigang;Li, Zhi;Dong, Zhimin;Zhang, Chunbao;Dong, Yingshan;Li, Yuqiu;Xia, Zhengjun;Zhang, Bo;Tadege, Million
关键词：soybean;
PRR ; rhythmic expression; interaction -
Precise mapping and candidate gene identification of the Rmd_JY47 locus conferring resistance to powdery mildew in soybean
作者：Sang, Yongsheng;Wang, Yumin;Liu, Xiaodong;Wen, Min;Dong, Lingchao;Qi, Guangxun;Yuan, Cuiping;Yang, Jiahui;Zhao, Hongkun;Dong, Yingshan;Sang, Yongsheng;Liu, Yue;Wen, Min;Zhang, Jian
关键词：Oil crop; Soybean; Powdery mildew; QTL; Candidate genes; MAS
-
Integration of induction, system optimization and genetic transformation in Veratrum californicum var. vitro cultures to enhance the production of cyclopamine and veratramine
作者：Zuo, Anqi;He, Di;Sun, Chongrui;Wen, Yashi;Li, He;Liu, Jingying;Ma, Pengda;Kou, Chengxi;Xue, Zheyong;Shao, Gaige;Ma, Rui;Wei, Jia
关键词：Veratrum californicum var.; Steroid alkaloid; Efficient production; VnOSCs
-
Efficient heterologous biosynthesis of verazine, a metabolic precursor of the anti-cancer drug cyclopamine, in Nicotiana benthamiana
作者：Kou, Chengxi;Liu, Jingling;He, Di;Ma, Pengda;Kou, Chengxi;Yin, Xue;Liu, Jia;Hua, Xin;Xue, Zheyong;Kou, Chengxi;Yin, Xue;Liu, Jia;Hua, Xin;Xue, Zheyong;Ma, Rui;Sun, Wei
关键词：
-
Genome-wide association study of powdery mildew resistance in cultivated soybean from Northeast China
作者：Sang, Yongsheng;Dong, Yingshan;Sang, Yongsheng;Zhao, Hongkun;Yuan, Cuiping;Qi, Guangxun;Li, Yuqiu;Dong, Lingchao;Wang, Yingnan;Wang, Yumin;Dong, Yingshan;Liu, Xiaodong;Wang, Dechun
关键词：soybean; powdery mildew; single nucleotide polymorphism; GWAS; candidate genes
-
Increased Accumulation of Recombinant Proteins in Soybean Seeds via the Combination Strategy of Polypeptide Fusion and Suppression of Endogenous Storage Proteins
作者：Yang, Jing;Zhang, Yuanyu;Xing, Guojie;Wei, Jia;Niu, Lu;Zhao, Qianqian;Cai, Qinan;Zhong, Xiaofang;Yang, Xiangdong
关键词：soybean; seed storage protein; RNAi; polypeptide fusion; GFP fusion
