Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells
文献类型： 外文期刊
作者： Guo, Rihong 1 ; Chen, Fang 2 ; Shi, Zhendan 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing 210014, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China
关键词： porcine; granulosa cells; progesterone; Notch signaling
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN：
年卷期： 2020 年 11 卷 2 期
页码：
收录情况： SCI
摘要： The conserved Notch pathway is reported to be involved in progesterone synthesis and secretion; however, the exact effects remain controversial. To determine the role and potential mechanisms of the Notch signaling pathway in progesterone biosynthesis in porcine granulosa cells (pGCs), we first used a pharmacological gamma-secretase inhibitor, N-(N-(3,5-difluorophenacetyl-l-alanyl))-S-phenylglycine t-butyl ester (DAPT), to block the Notch pathway in cultured pGCs and then evaluated the expression of genes in the progesterone biosynthesis pathway and key transcription factors (TFs) regulating steroidogenesis. We found that DAPT dose- and time-dependently increased progesterone secretion. The expression of steroidogenic proteins NPC1 and StAR and two TFs, NR5A2 and NR2F2, was significantly upregulated, while the expression of HSD3B was significantly downregulated. Furthermore, knockdown of both NR5A2 and NR2F2 with specific siRNAs blocked the upregulatory effects of DAPT on progesterone secretion and reversed the effects of DAPT on the expression of NPC1, StAR, and HSD3B. Moreover, knockdown of NR5A2 and NR2F2 stimulated the expression of Notch3. In conclusion, the inhibition of Notch signaling stimulated progesterone secretion by enhancing the expression of NPC1 and StAR, and the two TFs NR5A2 and NR2F2 acted as downstream TFs of Notch signaling in regulating progesterone synthesis.
- 相关文献
作者其他论文 更多>>
-
miR-21/SMAD2 Is Involved in the Decrease in Progesterone Synthesis Caused by Lipopolysaccharide Exposure in Follicular Granulosa Cells of Laying Goose
作者：Guo, Xinyi;Ying, Shijia;Xiao, Huiping;An, Hao;Wu, Wenda;Guo, Xinyi;Xiao, Huiping;Wu, Wenda;Guo, Xinyi;Ying, Shijia;An, Hao;Guo, Rihong;Dai, Zichun
关键词：lipopolysaccharide; granulosa cells; miR-21; progesterone; goose
-
Improving duckling hatchability and quality by optimization of egg turning angle during incubation
作者：Chen, Fang;Zhu, Biwen;Guo, Binbin;Dai, Zichun;Ying, Shijia;Shi, Zhendan;Chen, Fang;Zhu, Biwen;Guo, Binbin;Dai, Zichun;Ying, Shijia;Shi, Zhendan;Zhu, Biwen;Huang, Yunmao;Shi, Zhendan
关键词：duck; egg turning angle; incubation; hatchability; growth performance
-
Effects of ambient temperature on growth performance, slaughter traits, meat quality and serum antioxidant function in Pekin duck
作者：Xu, Congcong;Sun, Dongyue;Liu, Yi;Pan, Ziyi;Dai, Zichun;Chen, Fang;Guo, Rihong;Chen, Rong;Shi, Zhendan;Ying, Shijia;Xu, Congcong;Sun, Dongyue;Liu, Yi;Shi, Zhendan;Ying, Shijia
关键词：Pekin duck; temperature; growth performance; slaughtering traits; meat quality; antioxidant function
-
Genetically Engineered Goats as Efficient Mammary Gland Bioreactors for Production of Recombinant Human Neutrophil Peptide 1 Using CRISPR/Cas9
作者：Li, Dongxu;Wang, Jingang;Wang, Feng;Wan, Yongjie;Guo, Rihong;Chen, Fang;Guo, Rihong;Chen, Fang
关键词：CRISPR/Cas9; goat; HNP1; beta-casein; antibacterial activity
-
The role of miR-10a-5p in LPS-induced inhibition of progesterone synthesis in goose granulosa cells by down-regulating CYP11A1
作者：Guo, Xinyi;An, Hao;Ying, Shijia;Wu, Wenda;Guo, Xinyi;An, Hao;Guo, Rihong;Dai, Zichun;Ying, Shijia;Guo, Xinyi;Wu, Wenda
关键词：LPS; goose; miR-10a-5p; granulosa cells; progesterone
-
The Impact of Different Relative Humidity Levels on the Production Performance, Slaughter Performance, and Meat Quality of White Pekin Ducks Aged 4 to 42 Days
作者：Sun, Dongyue;Ying, Shijia;Sun, Dongyue;Xu, Congcong;Liu, Yi;Dai, Zichun;Pan, Ziyi;Chen, Rong;Guo, Rihong;Chen, Fang;Shi, Zhendan;Ying, Shijia;Xu, Congcong;Liu, Yi
关键词：Pekin ducks; production performance; meat quality; humidity
-
Bacterial lipopolysaccharide with different administration routes affects intestinal mucosal morphological, immunological, and microbial barrier functions in goslings
作者：Xi, Yumeng;Li, Yue;Ying, Shijia;Yan, Junshu;Shi, Zhendan
关键词：gosling; lipopolysaccharide challenge; intestinal mucosal barrier function
