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miR-21/SMAD2 Is Involved in the Decrease in Progesterone Synthesis Caused by Lipopolysaccharide Exposure in Follicular Granulosa Cells of Laying Goose

文献类型： 外文期刊

作者： Guo, Xinyi 1 ; Ying, Shijia 1 ; Xiao, Huiping 1 ; An, Hao 1 ; Guo, Rihong 3 ; Dai, Zichun 3 ; Wu, Wenda 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China

2.Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Anim Sci, Minist Agr & Rural Affairs, Key Lab Crop & Anim Integrated Farming, Nanjing 210014, Peoples R China

关键词： lipopolysaccharide; granulosa cells; miR-21; progesterone; goose

期刊名称：METABOLITES （ 影响因子：3.4； 五年影响因子：4.0 ）

ISSN：

年卷期： 2024 年 14 卷 7 期

页码：

收录情况： SCI

摘要： Lipopolysaccharide (LPS) is one of the important pathogenic substances of E. coli and Salmonella, which causes injury to the reproductive system. Ovarian dysfunction due to Gram-negative bacterial infections is a major cause of reduced reproductive performance in geese. However, the specific molecular mechanisms of LPS-induced impairment of sex steroid hormone synthesis have not been determined. The regulatory mechanism of miRNA has been proposed in many physiological and pathogenic mechanisms. Therefore, the role of miRNA in breeding geese exposed to LPS during the peak laying period was investigated. In this study, twenty Yangzhou geese at peak laying period were injected with LPS for 0 h, 24 h, and 36 h. The follicular granulosa layer was taken for RNA-seq and analyzed for differentially expressed miRNAs. It was observed that LPS changed the appearance of hierarchical follicles. miRNA sequencing analysis was applied, and miR-21 and SMAD2 (SMAD family member 2) were selected from 51 differentially expressed miRNAs through bioinformatics prediction. The results showed that miR-21 down-regulated SMAD2 expression and progesterone (P4) production in LPS-treated goose granulosa cells (GCs). It also determined that overexpression of miR-21 or silence of SMAD2 suppressed the sex steroid biosynthesis pathway by decreasing STAR and CYP11A1 expression. Down-regulation of miR-21 exacerbates the LPS-induced decline in P4 synthesis and vice versa. The findings indicated that miR-21 was involved in LPS regulation of P4 synthesis in goose granulosa cells by down-regulating SMAD2. This study provides theoretical support for the prevention of LPS-induced ovarian dysfunction in geese.

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