Green tea and black tea inhibit proliferation and migration of HepG2 cells via the PI3K/Akt and MMPs signalling pathway
文献类型： 外文期刊
作者： Sun, Lingli 1 ; Zhang, Yuanlong 2 ; Zhang, Wenji 1 ; Lai, Xingfei 1 ; Li, Qiuhua 1 ; Zhang, Lingyun 2 ; Sun, Shili 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Tea Res Inst, Guangdong Prov Key Lab Tea Plant Resources Innova, Guangzhou 510640, Peoples R China
2.South China Agr Univ, Coll Hort, Guangzhou 510642, Peoples R China
关键词： Tea; HepG2; Tumour proliferation; Tumour migration; PI3K/Akt pathway
期刊名称：BIOMEDICINE & PHARMACOTHERAPY （ 影响因子：6.529； 五年影响因子：5.979 ）
ISSN： 0753-3322
年卷期： 2020 年 125 卷
页码：
收录情况： SCI
摘要： Background and aims: Black tea and green tea were produced via different processing techniques from the same tea leave variety. Then, biochemical components of the two water extracts were analysed to study cell apoptosis, migration and invasion of HepG2 cells induced by black tea and green tea. Method: The monomer components of the black tea and green tea extracts were analysed by colorimetry and HPLC, with MTT assay and colony formation assays used to assess cell proliferation and viability. The effects of black tea and green tea on apoptosis of HepG2 cells were verified by flow cytometry, with wound healing and Transwell experiments used to detect cell invasion and metastasis. The expression of PI3K/Akt signalling and apoptosis-related proteins as well as epithelial-mesenchymal transition (EMT) regulatory factor in HepG2 cells were determined by western blotting after black tea and green tea treatment. Results and conclusions: Black tea and green tea extracts demonstrated different degrees of inhibition of cell migration and invasion, with green tea inducing more HepG2 cell apoptosis. In addition, green tea and black tea extracts inhibited the growth of HepG2 cells and induced apoptosis via PI3K/Akt, and inhibited cell migration and invasion through the MMPs signalling pathway. This study revealed the effects of fermented (black tea) and non-fermented tea (green tea) on liver cancer cells, providing a basis for the investigation of tea extracts for their anti-tumour potential.
- 相关文献
作者其他论文 更多>>
-
Mechanism of Green Tea Peptides in Lowering Blood Pressure and Alleviating Renal Injury Induced by Hypertension Through the Ang II/TGF-β1/SMAD Signaling Pathway
作者：Li, Lulu;Li, Dongli;Li, Lulu;Sun, Shili;Lai, Xingfei;Li, Qiuhua;Chen, Ruohong;Zhang, Zhenbiao;Hao, Mengjiao;Zhang, Suwan;Sun, Lingli;Li, Dongli
关键词：tea polypeptide; hypertension; renal injury; TGF-beta/Smad signaling
-
Metabolomics-based analysis of the effects of differences in soluble sugars on the sweetness quality of six major tea types in China
作者：Li, Wen;Chen, Zhongzheng;Zhang, Zhenbiao;Chen, Ruohong;Sun, Lingli;Lai, Xingfei;Li, Qiuhua;Hao, Mengjiao;Zhang, Suwang;Sun, Shili;Li, Qian
关键词：
-
The biological functions of proanthocyanidin and its application in pig production
作者：Yu, Aiying;Chen, Lian;Wang, Dan;Li, Zhiyi;Wang, Yuan;Wang, Zhenjiang;Lin, Sen;Sun, Lingli;Sun, Lingli
关键词：proanthocyanidins; biological functions; antioxidant; anti-inflammatory; pigs
-
Global m6A RNA and whole 5mC DNA methylation specifically contribute to cell replicative and premature senescence induced by extrinsic oxidative stress
作者：Zhu, Chenyu;Huang, Tingting;Fu, Jiaqi;Li, Min;Tan, Luyi;Zhang, Xinyu;Cheng, Wenli;Lai, Caiyun;Zhang, Wenjuan;Wang, Zhangying;Zhang, Wenji
关键词：H2O2; N6-methyladenosine; 5-methylcytosine; premature senescence; epigenetics
-
Effects of Pile-Fermentation Duration on the Taste Quality of Single-Cultivar Large-Leaf Dark Tea: Insights from Metabolomics and Microbiomics
作者：Yang, Wanying;Ni, He;Chen, Ruohong;Sun, Lingli;Li, Qiuhua;Lai, Xingfei;Zhang, Zhenbiao;Lai, Zhaoxiang;Hao, Mengjiao;Sun, Shili;Li, Qian;Lin, Sen
关键词：dark tea; quality; taste; pile-fermentation; metabolomics; microbiomics
-
Analysis of volatile metabolites and differences in aroma quality of green tea, black tea and oolong tea prepared from highly aromatic tea variety (Dancong)
作者：Zhang, Suwan;Xia, Hongling;Sun, Shili;Ma, Chenying;Pan, Shunshun;Zhang, Zhenbiao;Lai, Xingfei;Li, Qiuhua;Hao, Mengjiao;Sun, Lingli;Chen, Ruohong;Zhang, Suwan;Xia, Hongling;Sun, Shili;Ma, Chenying;Pan, Shunshun;Zhang, Zhenbiao;Lai, Xingfei;Li, Qiuhua;Hao, Mengjiao;Sun, Lingli;Chen, Ruohong;Li, Feng;Ma, Chenying
关键词：Fermentation degree; Tea; Aroma; VOCs; GC-MS
-
Inhibition of xanthine oxidase by copper-gallate coordination polymers and its mechanistic study
作者：Mo, Yejian;Ren, Jian;Sun, Shili;Shen, Xiaoyu;Mo, Yejian;Ren, Jian;Liu, Kexin;Deng, Chunlin;Shen, Xiaoyu;Mo, Yejian;Ren, Jian;Liu, Kexin;Deng, Chunlin
关键词：Hyperuricemia; Metal-polyphenol coordination polymers; Xanthine oxidase inhibitors; Antioxidant properties
