文献类型： 外文期刊

作者： Gong, A. D. ¹ ; Sun, G. J. ¹ ; Zhao, Z. Y. ⁴ ; Liao, Y. C. ¹ ; Zhang, J. B. ¹ ;

作者机构： 1.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China

2.Xinyang Normal Univ, Coll Life Sci, Xinyang 464000, Peoples R China

3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

4.Shanghai Acad Agr Sci, Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Shangh, Inst Agrofood Stand & Testing Technol, Shanghai 200031, Peoples R China

关键词： Aspergillus flavus; aflatoxin; post-harvest; Staphylococcus saprophyticus; volatile organic compound; 3,3-dimethyl-1,2-epoxybutane

期刊名称：WORLD MYCOTOXIN JOURNAL （ 影响因子：3.353； 五年影响因子：3.108 ）

ISSN： 1875-0710

年卷期： 2020 年 13 卷 2 期

页码：

收录情况： SCI

摘要： Controlling proliferation and aflatoxin production by Aspergillus flavus is a pressing challenge for global food safety and security. Marine bacterium Staphylococcus saprophyticus strain L-38 showed excellent antifungal activity toward A. flavus in vitro and in vivo. In sealed, non-contact confrontation assays, L-38 completely inhibited conidial germination and mycelial growth of A. flavus through the production of volatile organic compounds (VOCs). Gas chromatography-mass spectrometry identified 3,3-dimethyl-1,2-epoxybutane (3-DE) as the most abundant VOC (32.61% of total peak area, 78% matching). Exposure of A. flavus cultures to synthetic 3-DE similarly demonstrated strong inhibition of growth. Moreover, culture of L-38 in a sealed chamber with maize or peanuts artificially inoculated with A. flavus, at high water activity, resulted in significant inhibition of A. flavus germination and aflatoxin biosynthesis. Scanning electron microscopy of these samples revealed severe damage to conidial cells and hyphae compared to samples not exposed to L-38. L-38 also showed broad and effective antifungal activity toward eight other phytopathogenic fungi including Aspergillus niger, Fusarium verticillioides, Fusarium graminearum, Sclerotinia sclerotiorum, Rhizoctonia solani, Alternaria alternata, Monilinia fructicola, and Botrytis cinerea. This work introduces S. saprophyticus L-38 as a potential biocontrol agent and demonstrates the efficacy of the volatile 3-DE in the control of A. flavus and other destructive plant pathogens for post-harvest food safety.