A new preparative method for simultaneous purification of ochratoxin A and ochratoxin B from wheat culture inoculated with Aspergillus ochraceus
文献类型: 外文期刊
作者: Zhao, Z. Y. 1 ; Liu, N. 2 ; Yang, L. C. 1 ; Wu, A. B. 2 ; Zhou, Z. L. 1 ; Deng, Y. F. 1 ; Song, S. Q. 3 ; Wang, J. H.; Ho 1 ;
作者机构: 1.Nanjing Agr Univ, Coll Vet Med, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Lab Qual & Safety Risk Assessment Agroprod Shangh, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
3.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Lab Qual & Safety Risk Assessment Ag
关键词: mycotoxin;ochratoxin;purification;acid-assisted liquid-liquid extraction;gel permeation chromatography
期刊名称:WORLD MYCOTOXIN JOURNAL ( 影响因子:3.353; 五年影响因子:3.108 )
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收录情况: SCI
摘要: Ochratoxins are a mycotoxin family frequently found in agricultural commodities worldwide and pose a potential health risk to humans and animals. To obtain large amounts of high-purity ochratoxins for food safety monitoring and toxicological research, a novel and effective method was established for simultaneous purification of ochratoxin A (OTA) and ochratoxin B (OTB) from a wheat culture inoculated with an ochratoxin-producing Aspergillus strain. The inoculated wheat culture was first extracted with methanol: water (80:20, v/v), followed by one or two cleanup procedures involving acid-assisted liquid-liquid extraction and gel permeation chromatography. Subsequently, target analytes were separated and collected using preparative high performance liquid chromatography. Finally, a combined approach of ultra-high performance liquid chromatography, ultraviolet spectrophotometry and mass spectrometry was applied for purity analysis and structural identification of the obtained toxins. As a result, 100 g of an inoculated wheat culture yielded 69 mg of OTA and 6 mg of OTB with purities greater than 98%. This proposed method might serve as a valuable reference to obtain expensive ochratoxin standards. To the best of our knowledge, this is the first report on simultaneous preparation of OTA and OTB from artificially inoculated wheat culture.
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