Using environmental DNA to detect Hypophthalmichthys molitrix during the spawning period in the Yangtze River
文献类型： 外文期刊
作者： Ruan, Rui 1 ; Wang, Dengqiang 2 ; Yue, Huamei 1 ; Li, Chuangju 1 ; Chen, Daqing 2 ; Duan, Xinbin 2 ;
作者机构： 1.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Freshwater Fish Germplasm & Biotechnol, Minist Agr, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
关键词： eDNA; Hypophthalmichthys molitrix; Yangtze River
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）
ISSN： 1877-7252
年卷期： 2020 年 12 卷 1 期
页码：
收录情况： SCI
摘要： The silver carp, Hypophthalmichthys molitrix, is one of four carps farmed in China, and its natural resources have been critically declining in the middle and upper reaches of the Yangtze River. Here, we applied the environmental DNA (eDNA) methodology to detect silver carp during the spawning period in the Yangtze River to supplement traditional survey techniques for determining spawning grounds and the scale of breeding. The results showed that the variations in the concentration of silver carp eDNA were detected by quantitative PCR assay, and also suggested that its genetic diversity could be rapidly monitored using the eDNA method.
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