Molecular characterization, expression and enzyme activity of three glutathione S-transferase genes from Eriocheir sinensis under pesticide stresses
文献类型： 外文期刊
作者： Zhang, Xiao 1 ; Shen, Guoqing 1 ; Wang, Yang 1 ; Huang, Pengdan 1 ; Ame, Kassimu Hashim 1 ; Zang, Yanan 1 ; Shen, Huai 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Nanjing 210095, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Jiangsu, Peoples R China
关键词： Eriocheir sinensis; GSTs; Pesticide stress; Gene expression; Metabolic detoxification
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：3.228； 五年影响因子：3.289 ）
ISSN： 1532-0456
年卷期： 2020 年 230 卷
页码：
收录情况： SCI
摘要： Glutathione S-transferases (GSTs) are a multifunctional protein superfamily that can catalyze the detoxification processes in an organism. In the present study, we determined the structure and function of GSTs in Chinese mitten crab (Eriocheir sinensis) by gene cloning, expression, and enzyme activity in order to investigate the metabolic detoxification of GSTs in the hepatopancreas and muscles under three pesticide (trichlorfon, beta-cypermethrin and avermectin) stresses. Multiple sequence alignment analysis showed that all the three Es-GST genes possessed N-terminal, and C-terminal domain as well as G-binding sites, while Es-GST2 and Es-GST3 contained Mu-type GST-specific Mu-loop structures. Phylogenetic tree analysis revealed that the three Es-GSTs belonged to the Mu-type GST of crustaceans. The quantitative real-time PCR revealed that the three Es-GSTS were expressed in 9 tissues of Eriocheir sinensis, with highest expression in hepatopancreas and muscle. The expression of the three Es-GSTS significantly increased in the hepatopancreas and muscle under the three pesticide stresses compared to the control group, and a steady increase in GST activity was observed. The study showed that the three Es-GSTs belong to the Mu-type GST of the crustaceans and might play an important role in the metabolic detoxification in Eriocheir sinensis.
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