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Transcriptomic analysis reveals different responses to ammonia stress and subsequent recovery between Coilia nasus larvae and juveniles

文献类型： 外文期刊

作者： Gao, Jun 1 ; Zhu, Yongxiang 3 ; Guo, Zhenglong 3 ; Xu, Gangchun 1 ; Xu, Pao 1 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Jiangsu, Peoples R China

3.Nantong Longyang Aquat Prod Co Ltd, Nantong 226600, Peoples R China

关键词： Transcriptomic analysis; Ammonia stress and recovery; Coilia nasus larvae and juveniles; Purine metabolism; Epigenetic modifications

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：3.228； 五年影响因子：3.289 ）

ISSN： 1532-0456

年卷期： 2020 年 230 卷

页码：

收录情况： SCI

摘要： Excessive ammonia triggered negative effects on aquatic animals' health, growth, and mass death, especially at different developmental periods. However, the underlying responses to ammonia stress in fish larvae and juveniles were much less explored. Transcriptomic analysis of Coilia nasus larvae and juveniles treated with ammonia stress and subsequent recovery in freshwater were performed. Total 958,213,132 clean reads were obtained. A total of 234,830 unigenes with an average length of 1397 bp and N50 value 2521 bp were assembled. 831 and 952 DEGs were identified in C. nasus larvae and juveniles, respectively. Transcriptomic analysis revealed that genes associated with purine metabolism, immune, inflammation, epigenetic modification, and nerve conduction presented different expression trends between C. nasus larvae and juveniles. Other genes related to purine metabolism (XDH) and epigenetic modifications (DNMT1, DNMT3A, and DNMT3B) detected by RT-qPCR also displayed different expression trends. These results indicated that ammonia detoxify strategies and gene regulation patterns were different in C. nasus larvae and juveniles. Higher TNF-alpha, ILF-2, and ILF-3 expression and reduced LZM, AKP, and ACP activities suggested that inflammation and declined immunity were triggered by ammonia stress. Additionally, nervous conduction was severely affected under ammonia stress in C. nasus juveniles. Furthermore, recovery in freshwater had positive effects on nervous conduction. However, it was worth noting that reduced immunity and inflammation were still existed after recovery in freshwater. In conclusion, our study would be beneficial to reveal the different responses to ammonia stress between larvae and juveniles.

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