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A novel gold particle-based paper sensor for sensitively detecting carprofen in bovine muscle

文献类型： 外文期刊

作者： Na, Guanqiong 1 ; Hu, Xiaofei 1 ; Sun, Yaning 1 ; Xing, Guangxu 1 ; Xing, Yunrui 1 ; Zhang, Gaiping 1 ;

作者机构： 1.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

4.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Carprofen; immunochromatography assay; monoclonal antibody; rapid detection

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2020 年 31 卷 1 期

页码：

收录情况： SCI

摘要： Carprofen (CAR) is a non-steroidal anti-inflammatory drug commonly used to treat various musculoskeletal diseases and infections in food animals. While CAR residues in food animal tissues caused by veterinary medicine abuse are a potential danger to human health. In this study, a high-affinity monoclonal antibody (mAb) against CAR was prepared, and then an immunochromatographic strip for rapidly screening CAR in bovine muscle was developed to help enforce regulatory compliance. The visual detection limit (cut-off value) by the naked eye of the fabricated test strip was 12.5 ng g(-1). Scanning by the strip reader, the IC50 of the test strip was calculated as 1.743 ng g(-1), and the limit of detection (LOD, IC10) was 0.283 ng g(-1). The proposed method provided an efficient tool for monitoring CAR in bovine muscle. Meantime, our work suggested great potential for the analysis of CAR residues in other food matrixes.

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