Cell wall surface layer (S-layer) promotes colony formation in Microcystis: comparison of S-layer characteristics between colonial and unicellular forms of Microcystis and function conformation
文献类型： 外文期刊
作者： Zu, Yao 1 ; Hong, Sujuan 1 ; Xu, Chongxin 1 ; Li, Weiwei 1 ; Chen, Siyu 1 ; Li, Jianhong 1 ;
作者机构： 1.Nanjing Normal Univ, Sch Life Sci, Nanjing 210046, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China
关键词： S-layer; Colony; Unicellular; Microcystis; Synechocystis sp; PCC6803; Cell wall
期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）
ISSN： 0944-1344
年卷期：
页码：
收录情况： SCI
摘要： Colony is a key to Microcystis becoming a dominant population and forming blooms. To find the mechanism of colony formation, we investigated cell wall structures of colonial and unicellular strains. Results showed that colonial strains had significant surface layer protein (S-layer) on the surface of cells than unicellular strains by transmission electron microscopy. Western blot showed colonial strains had more S-layer than the unicellular strains. When the S-layer gene (GenBank accession number CAO89090.1) of Microcystis aeruginosa PCC7806 was expressed in Synechocystis sp. PCC6803, PCC6803 aggregated into colonial morphology. The results indicated that the S-layer could promote colony formation in Microcystis. Based on the S-layer sequences of PCC6803 and PCC7806, nine S-layer genes in other Microcystis strains were screened from the GenBank. Sequence comparing showed that the S-layers conserved regions were all located in N-terminal. The S-layers contain repeats-in-toxin (RTX) sequences with Ca2+-binding site, and their amino acid composition, hydrophobicity, isoelectric point, etc. were consistent with the characteristics of RTX-type S-layer in bacteria.
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