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BIOLOGICAL EFFECTS OF 7LITHIUM ((LI)-L-7) ION BEAM RADIATION ON MUTATION INDUCTION IN CAPSICUM ANNUUM L.

文献类型： 外文期刊

作者： Wang, Xue 1 ; Xie, Libo 1 ; Liu, Luxiang 2 ; Chen, Lixin 1 ; Zhang, Hui 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Hort Subacad, Harbin, Heilongjiang, Peoples R China

2.Chinese Acad Agr Sci, Natl Ctr Space Mutagenesis Crop Improvement, Inst Crop Sci, Beijing, Peoples R China

关键词： Capsicum annuum L.; Li-7 ion beam; Mutant; SRAP

期刊名称：PAKISTAN JOURNAL OF BOTANY （ 影响因子：0.972； 五年影响因子：0.988 ）

ISSN： 0556-3321

年卷期： 2020 年 52 卷 3 期

页码：

收录情况： SCI

摘要： Lithium-7(Li-7) ion beam radiation is an effective method for inducing mutations in plants. In this study, the effects of Li-7 ion beam radiation on mutation induction in pepper (Capsicum annuum L.) were evaluated. The germination rates of irradiated seeds, survival rates of M1 plants and mutation frequencies of M2 plants were statistically analyzed. The results showed that these characteristics were all affected by radiation. A radiation dose of 60 Gy was the balance point between the survival rate of M1 plants and mutation rate of M2 plants, indicating that 60 Gy was a good choice for Li-7 ion beam radiation applications in breeding. Sequence-related amplified polymorphism (SRAP) analysis of select M2 plants indicated that radiation caused changes in the DNA. Observations of mutant morphology showed that useful mutants were generated from M2 plants. These results provide both a basis for the application of ion beam radiation in breeding and useful materials for studies of gene function.

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