您好，欢迎访问新疆农业科学院 机构知识库！
新疆农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

iTRAQ-Based Proteomics Reveals that the Tomato ms10(35) Gene Causes Male Sterility through Compromising Fat Acid Metabolism

文献类型： 外文期刊

作者： Wang, Baike 1 ; Li, Ning 1 ; Wang, Juan 1 ; Huang, Shaoyong 2 ; Tang, Yaping 1 ; Yang, Shengbao 1 ; Yang, Tao 1 ; Wang, Q 1 ;

作者机构： 1.Xinjiang Acad Agr Sci, Inst Hort Crops, Urumqi 830091, Peoples R China

2.Xinjiang Agr Univ, Coll Forestry & Hort, Urumqi 830052, Peoples R China

关键词： absolute quantification; genetic male sterility; isobaric tags; regulation network; relative quantification; tomato (Solanum lycoperscium)

期刊名称：PROTEOMICS （ 影响因子：3.984； 五年影响因子：3.831 ）

ISSN： 1615-9853

年卷期：

页码：

收录情况： SCI

摘要： So far, over 50 spontaneous male sterile mutants of tomato have been described and most of them are categorized as genetic male sterility. To date, the mechanism of tomato genetic male sterility remained unclear. In this study, differential proteomic analysis is performed between genetic male sterile line (2-517), which carries the male sterility (ms10(35)) gene, and its wild-type (VF-11) using isobaric tags for relative and absolute quantification-based strategy. A total of 8272 proteins are quantified in the 2-517 and VF-11 lines at the floral bud and florescence stages. These proteins are involved in different cellular and metabolic processes, which express obvious functional tendencies toward the hydroxylation of the omega-carbon in fatty acids, the tricarboxylic acid cycle, the glycolytic, and pentose phosphate pathways. Based on the results, a protein network explaining the mechanisms of tomato genetic male sterility is proposed, finding the compromising fat acid metabolism may cause the male sterility. These results are confirmed by parallel reaction monitoring, quantitative Real-time PCR (qRT-PCR), and physiological assays. Taken together, these results provide new insights into the metabolic pathway of anther abortion induced by ms10(35) and offer useful clues to identify the crucial proteins involved in genetic male sterility in tomato.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台