Biochemical Characterization of a New Oligoalginate Lyase and Its Biotechnological Application in Laminaria japonica Degradation
文献类型： 外文期刊
作者： Li, Shangyong 1 ; Wang, Linna 3 ; Jung, Samil 2 ; Lee, Beom Suk 2 ; He, Ningning 1 ; Lee, Myeong-Sok 2 ;
作者机构： 1.Qingdao Univ, Sch Basic Med, Qingdao, Peoples R China
2.Sookmyung Womens Univ, Cellular Heterogene Res Ctr, Dept Biosyst, Mol Canc Biol Lab, Seoul, South Korea
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
关键词： oligoalginate lyase; substrate specificity; alginate monomers; Laminaria japonica; Vibrio sp; SY01
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： Oligoalginate lyases catalyze the degradation of alginate polymers and oligomers into monomers, a prerequisite for biotechnological utilizing alginate. In this study, we report the cloning, expression and biochemical characterization of a new polysaccharide lyase (PL) family 17 oligoalginate lyase, OalV17, from the marine bacterium Vibrio sp. SY01. The recombinant OalV17 showed metal ion independent and detergent resistant properties. Furthermore, OalV17 is an exo-type enzyme that yields alginate monomers as the main product and recognizes alginate disaccharides as the minimal substrate. Site-directed mutagenesis followed by kinetic analysis indicates that the residue Arg(231) plays a key role in substrate specificity. Furthermore, a rapid and efficient alginate monomer-producing method was developed directly from Laminaria japonica. These results suggest that OalV17 is a potential candidate for saccharification of alginate.
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