Effects of an Escherichia coli exopolysaccharide on human and mouse gut microbiota in vitro
文献类型： 外文期刊
作者： Li, Baiyuan 1 ; Chen, Huahai 1 ; Cao, Linyan 1 ; Hu, Yunfei 1 ; Chen, Dan 1 ; Yin, Yeshi 1 ;
作者机构： 1.Hunan Univ Sci & Engn, Coll Chem & Bioengn, Key Lab Comprehens Utilizat Advantage Plants Reso, Yongzhou, Hunan, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou, Zhejiang, Peoples R China
关键词： EPS-RB; Gut microbiota; High-throughput sequencing; Short-chain fatty acid
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2020 年 150 卷
页码：
收录情况： SCI
摘要： In this study, an exopolysaccharide (EPS) named EPS-RB was produced when the gene cluster ycjD-fabl-yciW-rnb were overexpressed in E. coli. Monosaccharide composition analysis revealed that EPS-RB is a novel EPS that consisted of L-fucose, L-arabinose, D-galactose/N-acetyl glucosamine, D-glucose, D-xylose, D-ribose, and D-glucuronic add, and their molecular ratio was approximately 80:3:53:69:1:2:64. The content of carbohydrates, protein, and uronic adds in EPS-RB was 90.35 +/- 135%, 2.62 +/- 0.05% and 8.16 +/- 1.00%, respectively. The interaction between EPS-RB and gut microbiota was investigated using an in vitro batch fermentation system. The results showed that -96% of EPS-RB can be degraded by human fecal microbiota after 72 h fermentation, but few can be degraded by mouse cecal microbiota. Furthermore, high-throughput sequencing showed that EPS-RB regulates the human gut microbiota. The genera Collinsella, Butyricimonas, and Hafnia were enriched in group VIR (EPS-RB as a carbon source) when compared with group VI (no carbon source) and VIS (starch as a carbon source). Short-chain fatty acids (SCFAs) production analysis showed that their concentration was significantly higher in group VIR than groups VI and VIS after 72 h fermentation. In summary, an EPS-RB in E. coli was isolated and its regulatory function on gut microbiota was analyzed. (C) 2019 Elsevier B.V. All rights reserved.
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