A staining method for detection of Enterocytozoon hepatopenaei (EHP) spores with calcofluor white
文献类型： 外文期刊
作者： Zhao, Ruo-Heng 2 ; Gao, Wen 1 ; Qiu, Liang 2 ; Chen, Xing 2 ; Dong, Xuan 2 ; Li, Chen 2 ; Huang, Jie 1 ;
作者机构： 1.Shanghai Ocean Univ, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst,Qingdao Key Lab Mar, Lab Marine Fisheries Sci & Food Prod Proc,Minist, Pilot Natl Lab Marine Sci & Technol Qingdao,Key L, Qingdao 266071, Peoples R China
关键词： Enterocytozoon hepatopenaei; Calcofluor white stain; Histopathology; Fluorescence
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）
ISSN： 0022-2011
年卷期： 2020 年 172 卷
页码：
收录情况： SCI
摘要： A fluorescent stain, calcofluor white (CFW), was used for detection of spores of the microsporidium Enterocytozoon hepatopenaei (EHP). EHP spores in suspension, in feces, or in the infected hepatopancreas of the shrimp Penaeus vannamei, can be easily stained with this chitin and cellulose binding dye to show distinct blue-white fluorescent oval walls. The dye does not stain the host tissues. EHP spores showed orange-red spots by staining with hematoxylin and phloxine (H&P) in the section. CFW staining provides a simple and rapid method for determining the presence of EHP spores in fecal or tissue samples.
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