A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain
文献类型： 外文期刊
作者： He, Wei-Jie 1 ; Shi, Meng-Meng 1 ; Yang, Peng 1 ; Huang, Tao 1 ; Zhao, Yue 1 ; Wu, Ai-Bo 5 ; Dong, Wu-Bei 3 ; Li, He-Ping 1 ;
作者机构： 1.Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China
2.Hubei Acad Agr Sci, Hubei Engn & Technol Res Ctr Wheat, Food Crops Inst, Wheat Dis Biol Res Stn Cent China, Wuhan 430064, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
4.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Food Safety Res Inst Nutr Sci, Shanghai 200031, Peoples R China
关键词： Deoxynivalenol epimerization; Enzymatic detoxification; Alcohol dehydrogenase; Aldo/keto reductases; Molecular docking
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2020 年 321 卷
页码：
收录情况： SCI
摘要： The Fusarium mycotoxin deoxynivalenol (DON) is typically controlled by fungicides. Here, we report DON detoxification using enzymes from the highly active Devosia strain D6-9 which degraded DON M 2.5 mu g/min/10(8) cells. Strain D6-9 catabolized DON to 3-keto-DON and 3-epi-DON, completely removing DON in wheat. Genome analysis of three Devosia strains (D6-9, D17, and D13584), with strain D6-9 transcriptomes, identified three genes responsible for DON epimerization. One gene encodes a quinone-dependent DON dehydrogenase QDDH which oxidized DON into 3-keto-DON. Two genes encode the NADPH-dependent aldo/keto reductases AKR13B2 and AKR6D1 that convert 3-keto-DON into 3-epi-DON. Recombinant proteins expressed in Escherichia coll. efficiently degraded DON in wheat grains. Molecular docking and site-directed mutagenesis revealed that residues S497, E499, and E535 function in QDDH's DON-oxidizing activity. These results advance potential microbial and enzymatic elimination of DON in agricultural samples and lend insight into the underlying mechanisms and molecular evolution of DON detoxification.
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