文献类型： 外文期刊

作者： Li Bei ¹ ; He Xiaolong ² ; Zhao Yiping ¹ ; Bai Dongyi ¹ ; Li Dandan ¹ ; Zhou Zhiyu ¹ ; Manglai, Dugarjaviin ¹ ;

作者机构： 1.Inner Mongolia Agr Univ, Inner Mongolia Key Lab Equine Genet Breeding & Re, Sci Observing & Expt Stn Equine Genet Breeding &, Minist Agr & Rural Affairs,Equine Res Ctr,Coll An, Zhaowuda Rd 306, Hohhot, Inner Mongolia, Peoples R China

2.Inner Mongolia Acad Agr & Anim Husb Sci, Zhaojun Rd 22, Hohhot, Inner Mongolia, Peoples R China

关键词： equine; gametogenesis; non-coding RNA; reproductive development

期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：2.311； 五年影响因子：2.396 ）

ISSN： 1031-3613

年卷期： 2020 年 32 卷 6 期

页码：

收录情况： SCI

摘要： Numerous studies have shown that microRNAs (miRNAs) are essential for testicular development and spermatogenesis. In order to further characterise these physiological processes, three immature and three mature testes of the Mongolian horse were collected and six libraries were established. Using small RNA sequencing technology, 531 mature miRNAs were identified, including 46 novel miRNAs without previously ascribed functions. Among the 531 miRNAs, 421 were expressed in both immature and mature libraries, 65 miRNAs were found solely in immature testis libraries and 45 miRNAs were found solely in mature testis libraries. Furthermore, among the miRNAs that were identified in both immature and mature libraries, 107 were significantly differentially expressed (corrected P value (padj) < 0.05). Among the miRNAs that were only expressed in immature testes, two miRNAs were differentially expressed, whereas among the miRNAs that were only expressed in mature testes, nine miRNAs were differentially expressed. Comprehensive analysis of miRNA and mRNA expression profiles predicted 107 miRNA-mRNA interaction sites. Gene ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis of the predicted target genes suggested roles of the differentially expressed miRNAs in testicular development and spermatogenesis. These findings identify miRNAs as key factors in the development of the testes and spermatogenesis in the Mongolian horse, which may also help us to understand the mechanisms of fertility in related mammalian species.