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Succession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose

文献类型： 外文期刊

作者： van Munster, Jolanda M. 1 ; Daly, Paul 1 ; Blythe, Martin J. 6 ; Ibbett, Roger 7 ; Kokolski, Matt 1 ; Gaddipati, Sa 1 ;

作者机构： 1.Univ Nottingham, Sch Life Sci, Univ Pk, Nottingham NG7 2RD, England

2.Univ Manchester, MIB, Manchester M1 7DN, Lancs, England

3.Univ Manchester, Sch Chem, Manchester M1 7DN, Lancs, England

4.Univ Utrecht, Westerdijk Fungal Biodivers Inst, Fungal Physiol, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

5.Univ Utrecht, Fungal Mol Physiol, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

6.Univ Nottingham, Queens Med Ctr, Fac Med & Hlth Sci, Deep Seq, Nottingham NG7 2UH, England

7.Univ Nottingham, Sch Biosci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England

8.US DOE, Joint Genome Inst, Lawrence Berkeley Natl Lab, Berkeley, CA 94598 USA

9.VTT Tech Res Ctr Finland, Tietotie 2, Espoo 02044, Finland

10.Publ Hlth England, Natl Infect Serv Salisbury, Salisbury, Wilts, England

11.Sorbonne Univ, CNRS, Inst Biol Paris Seine, Lab Computat & Quantitat Biol, F-75005 Paris, France

12.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China

关键词： Aspergillus niger; Lignocellulose; Pretreatment; System analysis; CAZymes; Metabolism; Fatty acids beta-oxidation; farA; Secondary metabolism

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN：

年卷期： 2020 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Background Understanding how fungi degrade lignocellulose is a cornerstone of improving renewables-based biotechnology, in particular for the production of hydrolytic enzymes. Considerable progress has been made in investigating fungal degradation during time-points where CAZyme expression peaks. However, a robust understanding of the fungal survival strategies over its life time on lignocellulose is thereby missed. Here we aimed to uncover the physiological responses of the biotechnological workhorse and enzyme producer Aspergillus niger over its life time to six substrates important for biofuel production. Results We analysed the response of A. niger to the feedstock Miscanthus and compared it with our previous study on wheat straw, alone or in combination with hydrothermal or ionic liquid feedstock pretreatments. Conserved (substrate-independent) metabolic responses as well as those affected by pretreatment and feedstock were identified via multivariate analysis of genome-wide transcriptomics combined with targeted transcript and protein analyses and mapping to a metabolic model. Initial exposure to all substrates increased fatty acid beta-oxidation and lipid metabolism transcripts. In a strain carrying a deletion of the ortholog of the Aspergillus nidulans fatty acid beta-oxidation transcriptional regulator farA, there was a reduction in expression of selected lignocellulose degradative CAZyme-encoding genes suggesting that beta-oxidation contributes to adaptation to lignocellulose. Mannan degradation expression was wheat straw feedstock-dependent and pectin degradation was higher on the untreated substrates. In the later life stages, known and novel secondary metabolite gene clusters were activated, which are of high interest due to their potential to synthesize bioactive compounds. Conclusion In this study, which includes the first transcriptional response of Aspergilli to Miscanthus, we highlighted that life time as well as substrate composition and structure (via variations in pretreatment and feedstock) influence the fungal responses to lignocellulose. We also demonstrated that the fungal response contains physiological stages that are conserved across substrates and are typically found outside of the conditions with high CAZyme expression, as exemplified by the stages that are dominated by lipid and secondary metabolism.

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