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Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to Powdery Mildew

文献类型： 外文期刊

作者： Li, Lanjun 1 ; Zhao, Degang 1 ;

作者机构： 1.Guizhou Univ, Inst Agr Bioengn, Key Lab Plant Resource Conservat & Germplasm Innov, Minist Educ,Coll Life Sci, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Biotechnol Inst Guizhou, Plant Conservat Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China

关键词： Eucommia ulmoides Oliv; EuCHIT30.7; powdery mildew; chitinase; tobacco

期刊名称：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：1.7； 五年影响因子：1.4 ）

ISSN： 0031-9457

年卷期： 2023 年 92 卷 11 期

页码：

收录情况： SCI

摘要： Various strains of powdery mildew (PM), a notorious plant fungal disease, are prevalent and pose a significant threat to plant health. To control PM, transgenic technology can be used to cultivate more resistant plant varieties. In the present study, we utilized the rapid amplification of cDNA ends (RACE) technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance functions. Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase. The EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant, with the most abundant expression in male flowers. Subcellular localization analysis indicated that the protein encoded by this gene was detected within both the cell membrane and cytoplasm. Upon PM inoculation, overexpression of EuCHIT30.7 in tobacco plants led to a significantly reduced relative lesion area and a decreased spore count compared to both wild-type and empty vector control plants. Activities of the protective enzymes, namely, peroxidase (POD), superoxide dis-mutase (SOD), catalase (CAT), and phenylalaninammo-nialyase (PAL), in tobacco plants overexpressing EuCHIT30.7 were significantly greater than those in wild-type and empty vector tobacco plants. Furthermore, the rate of increase in malondialdehyde (MDA) content was significantly lower in tobacco plants expressing EuCHIT30.7 compared to control tobacco plants. In EuCHIT30.7 transgenic tobacco, the expression of patho-gen-related protein genes, namely, PR2, PR5, PR1a, PDF1.2, and MLP423, along with the tobacco PM negative regulatory gene, MLO2, were significantly higher compared to control tobacco plants. These findings suggested that EuCHIT30.7 significantly enhances the resistance of tobacco to PM.

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