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Molecular cloning, characterization and expression analysis of CmAPX

文献类型： 外文期刊

作者： Cheng, Hong 2 ; He, Qiwei 1 ; Huo, Yumeng 3 ; Hou, Lixia 1 ; Lv, Junfen 4 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Vegetable, Jinan 250100, Peoples R China

2.Gansu Acad Agr Sci, Inst Vegetable, Lanzhou 730070, Peoples R China

3.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China

4.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： The RT PCR and RACE methods were used to obtain the cDNA sequence of an APX gene of muskmelon after the leaves were induced with powdery mildew. The cDNA length of the APX gene is 1,047 bp with a 750 bp ORF encoded a 249 amino acid and the molecular weight of APX protein is 27.3 kDa. The analysis showed that the CmAPX genomic DNA contained 10 extrons and 9 introns. The identity of the amino acid sequence deduced from the cDNA with the APX family of other homologous members was about 74-97%. A Full-length of ORF was sub-cloned into prokaryotic expression vector pET24a. The recombinant proteins had high expression level in E. coli. Analysis of expression at mRNA level showed that CmAPX exhibited highly tissue-specific patterns of expression. The mRNA level and enzyme activities assays showed that CmAPX might play an important role in the pathogenesis of powdery mildew.

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