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Monitoring peroxisome dynamics using enhanced green fluorescent protein labeling in Alternaria alternata

文献类型： 外文期刊

作者： Lu, Ziqi 1 ; Guo, Jian 3 ; Li, Qiang 1 ; Han, Yatao 1 ; Zhang, Zhen 2 ; Hao, Zhongna 2 ; Wang, Yanli 2 ; Sun, Guochang 2 ; Wang, Jiaoyu 2 ; Li, Ling 1 ;

作者机构： 1.Zhejiang Agr & Forestry Univ, Coll Adv Agr Sci, Key Lab Qual Improvement Agr Prod Zhejiang Prov, Hangzhou, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

3.Zhejiang Agr & Forestry Univ, Coll Food & Hlth, Coll Modern Food Ind, Hangzhou, Peoples R China

关键词： Alternaria alternata; eGFP-PTS2; peroxisome; dynamic analysis; lipid

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Brown leaf spot on tobacco is a serious fungal disease caused by Alternaria alternata. Peroxisomes are organelles playing an important role in the development and infection of plant pathogenic fungi. But, until now, there is no report on the peroxisome dynamics during the conidia germination of A. alternata. To evaluate the roles of peroxisome in the development of the fungus, in the present work, an enhanced green fluorescent protein (eGFP) cassette tagged with peroxisome targeting signal 2 (PTS2) was integrated into A. alternata to label the organelles, and an eGFP cassette carrying a nuclear located signal (NLS) was performed parallelly. The transformants containing the fusions emitted fluorescence in punctate patterns. The fluorescence of eGFP-PTS2 was distributed exactly in the peroxisomes while those of eGFP-NLS were located in the nucleus. Typical AaGB transformants were selected to be investigated for the peroxisome dynamics. The results showed that during spore germination, the number of peroxisomes in the spores decreased gradually, but increased in the germ tubes. In addition, when the transformants were cultured on lipid media, the numbers of peroxisomes increased significantly, and in a larger portion, present in striped shapes. These findings give some clues for understanding the peroxisomal functions in the development of A. alternata.

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