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Antioxidant activities of different fractions of polysaccharide purified from pulp tissue of litchi (Litchi chinensis Sonn.)

文献类型： 外文期刊

作者： Kong, Fan-Li 1 ; Zhang, Ming-Wei 1 ; Kuang, Rui-Bin 3 ; Yu, Shu-Juan 2 ; Chi, Jian-Wei 1 ; Wei, Zhen-Cheng 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Key Lab Funct Food, Minist Agr, Biotech Res Inst, Guangzhou 510610, Guangdong, Peoples R China

2.S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab Trop & Subtrop Fruit Trees, Guangzhou 510610, Guangdong, Peoples R China

关键词： Litchi;Polysaccharide;Purification;Antioxidant activity

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Three fractions of water-soluble polysaccharide fractions, coded as LFP1, LFP2 and LFP3, were isolated and purified from litchi pulp by DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel-permeation column. Their chemical and physical characteristics were determined by chemical methods, gas chromatography, 1R spectrophotometer and gel-permeation chromatography (GPC) with a model 410 refractive index detector. Antioxidant activities of these fractions were investigated using various in vitro assay systems. Results indicated that LFP1 was composed of arabinose, ribose, galactose and glucose, while LFP2 comprised arabinose, rhamnose and glucose. LFP3 was a heteropolysaccharide bounded with protein (2.6%) and composed of arabinose, rhamnose, galactose and glucose. The molecular weights of LFP1, LFP2 and LFP3 were 11.6 x 10~4,3.1 x 10~4 and 2.9 x 10~4 Da, respectively. LFP3 showed the highest antioxidant activities among the three fractions of polysaccharides, and could be explored as a novel potential antioxidant.

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