文献类型： 外文期刊

作者： Zhang, Jie ¹ ; He, Liheng ¹ ; Dong, Jingjing ¹ ; Zhao, Cailiang ¹ ; Wang, Yujie ³ ; Tang, Ruimin ⁴ ; Wang, Wenbin ⁴ ; Ji, Zhixian ⁵ ; Cao, Qinghe ⁶ ; Xie, Hong'e ⁷ ; Wu, Zongxin ⁷ ; Li, Runzhi ¹ ; Yuan, Ling ⁸ ; Jia, Xiaoyun ⁴ ;

作者机构： 1.Shanxi Agr Univ, Coll Agr, Jinzhong, Peoples R China

2.Changzhi Univ, Dept Life Sci, Changzhi, Peoples R China

3.Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, Henan Joint Int Lab Crop Multi Res, Kaifeng, Peoples R China

4.Shanxi Agr Univ, Coll Life Sci, Jinzhong, Peoples R China

5.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou, Peoples R China

6.Minist Agr, Xuzhou Inst Agr Sci, Xuzhou Sweetpotato Res Ctr, Key Lab Sweetpotato Biol & Genet Breeding, Xuzhou, Peoples R China

7.Shanxi Agr Univ, Inst Cotton Res, Yuncheng, Peoples R China

8.Univ Kentucky, Kentucky Tobacco Res & Dev Ctr, Dept Plant & Soil Sci, Lexington, KY USA

关键词： Ipomoea batatas; Gene expression; Carotenoids; Tuberous root development; Carotenoid cleavage dioxygenase 4(IbCCD4)

期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS

ISSN：

年卷期： 2023 年 16 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundPlant carotenoids are essential for human health, having wide uses in dietary supplements, food colorants, animal feed additives, and cosmetics. With the increasing demand for natural carotenoids, plant carotenoids have gained great interest in both academic and industry research worldwide. Orange-fleshed sweetpotato (Ipomoea batatas) enriched with carotenoids is an ideal feedstock for producing natural carotenoids. However, limited information is available regarding the molecular mechanism responsible for carotenoid metabolism in sweetpotato tuberous roots.ResultsIn this study, metabolic profiling of carotenoids and gene expression analysis were conducted at six tuberous root developmental stages of three sweetpotato varieties with different flesh colors. The correlations between the expression of carotenoid metabolic genes and carotenoid levels suggested that the carotenoid cleavage dioxygenase 4 (IbCCD4) and 9-cis-epoxycarotenoid cleavage dioxygenases 3 (IbNCED3) play important roles in the regulation of carotenoid contents in sweetpotato. Transgenic experiments confirmed that the total carotenoid content decreased in the tuberous roots of IbCCD4-overexpressing sweetpotato. In addition, IbCCD4 may be regulated by two stress-related transcription factors, IbWRKY20 and IbCBF2, implying that the carotenoid accumulation in sweeetpotato is possibly fine-tuned in responses to stress signals.ConclusionsA set of key genes were revealed to be responsible for carotenoid accumulation in sweetpotato, with IbCCD4 acts as a crucial player. Our findings provided new insights into carotenoid metabolism in sweetpotato tuberous roots and insinuated IbCCD4 to be a target gene in the development of new sweetpotato varieties with high carotenoid production.