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Production of all-male non-transgenic zebrafish by conditional primordial germ cell ablation

文献类型： 外文期刊

作者： Wang, Fang 1 ; Feng, Yong-Yong 2 ; Wang, Xu-Guang 3 ; Ou, Mi 1 ; Zhang, Xin-Cheng 1 ; Zhao, Jian 1 ; Chen, Kun-Ci 1 ; Li, Kai-Bin 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr, 1 Xingyu Rd, Guangzhou 510380, Guangdong, Peoples R China

2.Weifang Univ, Coll Seed & Facil Agr Engn, Weifang, Peoples R China

3.Guangdong Med Univ, Dept Orthoped, Affiliated Hosp, Zhanjiang 524001, Guangdong, Peoples R China

关键词： Primordial germ cell (PGC); Conditional removal; All-male population; Metronidazole

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0920-1742

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Many fish species exhibit remarkable sexual dimorphism, with males possessing numerous advantageous traits for commercial production by aquaculture such as faster growth rate, more efficient food energy utilization for muscle development, and better breeding performance. Several studies have shown that a decrease in the number of primordial germ cells (PGCs) during early development leads predominantly to male progeny. In this study, we developed a method to obtain all-male zebrafish (Danio rerio) by targeted PGC ablation using the nitroreductase/metronidazole (NTR/Mtz) system. Embryos generated by female heterozygous Tg(nanos3:nfsB-mCherry-nanos3 3 ' UTR) and male wild-types (WTs) were treated with vehicle or Mtz. Compared to vehicle-treated controls, 5.0 and 10.0 mM Mtz treatment for 24 h significantly reduced the number of PGCs and yielded an exclusively male phenotype in adulthood. The gonads of offspring treated with 5.0 mM Mtz exhibited relatively normal morphology and histological characteristics. Furthermore, these males were able to chase females, spawn, and produce viable offspring, while about 20.0% of males treated with 10.0 mM Mtz were unable to produce viable offspring. The 5.0 mM Mtz treatment protocol may thus be suitable for large-scale production of fertile male offspring. Moreover, about half of these males were WT as evidenced by the absence of nfsB gene expression. It may thus be possible to breed an all-male WT fish population by Mtz-mediated PGC ablation.

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