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Identification and characterization of the Enterobacter complex causing mulberry (Morus alba) wilt disease in China

文献类型： 外文期刊

作者： Wang, Guo-Fen 1 ; Xie, Guan-Lin 1 ; Zhu, Bo 1 ; Huang, Jun-Sheng 2 ; Liu, Bo 3 ; Kawicha, Praphat 4 ; Benyon, Lesley 5 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310029, Zhejiang, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Danzhou 571737, Hainan Province, Peoples R China

3.Fujian Acad Agr Sci, Agr Bioresource Res Inst, Fuzhou 350003, Fujian Province, Peoples R China

4.Kasetsart Univ, Fac Nat Resources & Agroind, Bangkok 47000, Sakonnakhon Pro, Thailand

5.USDA ARS USHRL, Ft Pierce, FL 34945 USA

关键词： gene sequence;metabolic profile;tree decline;tissue discoloration

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

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收录情况： SCI

摘要： Mulberry wilt disease (MWD) was recently identified in Hangzhou, Zhejiang province, China. Typical symptoms of the disease are browning of vascular tissues, leaf wilt, defoliation, and tree decline. Unlike the symptoms of bacterial wilt disease caused by Ralstonia solanacearum, symptoms of MWD generally started from the bottom of the plants and moved upward. In inoculation experiments, four selected MWD strains caused mulberry shoot leaf wilt, discoloration, and defoliation. They also induced whole plant leaf wilt, defoliation and dark brown discoloration of vascular tissue. Based on Biolog metabolic profiles, fatty acid methyl ester analysis (FAME) and sequence analysis of the partial 16S rDNA and rpoB genes four MWD strains were identified as members of the genus Enterobacter. The 16S rDNA and rpoB gene sequences revealed a close relationship among two isolates, R2-2 and R6-2, and the E. asburiae type strain JCM6051. The isolates showed >98% similarity to E. asburiae JCM6051 in their rpoB gene. These results indicated that isolates R2-2 and R6-2 belonged to E. asburiae. No similarity in 16S rDNA sequences above 97% was found between either of the remaining isolates, R11-2 or R18-2, and any recognized Enterobacter species, suggesting that the two isolates may represent novel Enterobacter species. rpoB gene similarity values between the isolates and Enterobacter spp. type strains were <98%, providing further evidence that the two isolates may represent a novel species within the Enterobacter. The causal agent for MWD was previously reported to be E. cloacae, however, this study found that other Enterobacter spp. (E. asburiae and Enterobacter sp.) also cause MWD.

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